Anti-TNP1 antibody (ab73135)
Key features and details
- Rabbit polyclonal to TNP1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TNP1 antibody
See all TNP1 primary antibodies -
Description
Rabbit polyclonal to TNP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Human normal testis FFPE section.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
In the elongating spermatids of mammals, the conversion of nucleosomal chromatin to the compact, non-nucleosomal form found in the sperm nucleus is associated with the appearance of a small set of basic chromosomal transition proteins. -
Tissue specificity
Testis. -
Sequence similarities
Belongs to the nuclear transition protein 1 family. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 7141 Human
- Entrez Gene: 21958 Mouse
- Entrez Gene: 100513764 Pig
- Entrez Gene: 24839 Rat
- Omim: 190231 Human
- SwissProt: P09430 Human
- SwissProt: P10856 Mouse
- SwissProt: P17306 Pig
see all -
Alternative names
- Spermatid nuclear transition protein 1 antibody
- Spermatid nuclear transition protein1 antibody
- STP 1 antibody
see all
Images
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IHC image of TNP1 staining in Human normal testis FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73135, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab73135 has been referenced in 12 publications.
- Merges GE et al. Loss of Prm1 leads to defective chromatin protamination, impaired PRM2 processing, reduced sperm motility and subfertility in male mice. Development 149:N/A (2022). PubMed: 35608054
- Wang M et al. Deciphering the autophagy regulatory network via single-cell transcriptome analysis reveals a requirement for autophagy homeostasis in spermatogenesis. Theranostics 11:5010-5027 (2021). PubMed: 33754041
- Kobayashi Y et al. Tsga8 is required for spermatid morphogenesis and male fertility in mice. Development 148:N/A (2021). PubMed: 33766931
- Tian P et al. Changes in Expressions of Spermatogenic Marker Genes and Spermatogenic Cell Population Caused by Stress. Front Endocrinol (Lausanne) 12:584125 (2021). PubMed: 34707565
- Miao J et al. MFN1 and MFN2 Are Dispensable for Sperm Development and Functions in Mice. Int J Mol Sci 22:N/A (2021). PubMed: 34948301