Product nameAnti-TNPO3 antibody
See all TNPO3 primary antibodies
DescriptionRabbit polyclonal to TNPO3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human, Bird
Predicted to work with: Cow, Xenopus laevis, Non human primates
Synthetic peptide corresponding to Human TNPO3 aa 100-200.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: Jurkat (Staurosporine Treated for 24hr, 500nM), Y79, Ramos, MEF1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab71388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 104 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionSeems to function in nuclear protein import as nuclear transport receptor. In vitro, mediates the nuclear import of splicing factor SR proteins SFRS1 and SFRS2, by recognizing phosphorylated RS domains.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- TNPO 3 antibody
- Imp12 antibody
- Importin 12 antibody
All lanes : Anti-TNPO3 antibody (ab71388) at 1 µg/ml
Lane 1 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 3 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab71388 stained MCF7 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71388, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% paraformaldehyde fixed (10 min) HeLa, Hek293 and HepG2 cells at 5µg/ml.
This product has been referenced in:
- De Iaco A et al. TNPO3 protects HIV-1 replication from CPSF6-mediated capsid stabilization in the host cell cytoplasm. Retrovirology 10:20 (2013). WB . Read more (PubMed: 23414560) »
- De Iaco A & Luban J Inhibition of HIV-1 infection by TNPO3 depletion is determined by capsid and detectable after viral cDNA enters the nucleus. Retrovirology 8:98 (2011). WB . Read more (PubMed: 22145813) »