Overview

  • Product name

    Anti-TNS4/CTEN antibody [SP83]
    See all TNS4/CTEN primary antibodies
  • Description

    Rabbit monoclonal [SP83] to TNS4/CTEN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TNS4/CTEN aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q8IZW8

  • Positive control

    • IHC-P: Human Liver Carcinoma, and Human hepatocellular carcinoma tissue; FC: HeLa.
  • General notes

     

     

     This product was previously labelled as TNS4

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab99887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    May be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton (By similarity). May promote apoptosis, via its cleavage by caspase-3.
  • Tissue specificity

    Prostate and placenta. Down regulated in prostate cancer.
  • Sequence similarities

    Contains 1 phosphatase tensin-type domain.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Proteolytically cleaved by caspase-3 during apoptosis.
  • Cellular localization

    Cell junction > focal adhesion. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • C terminal tensin like antibody
    • C terminal tensin like protein antibody
    • C-terminal tensin-like protein antibody
    • CTEN antibody
    • FLJ14950 antibody
    • PP14434 antibody
    • TENS4_HUMAN antibody
    • Tensin 4 antibody
    • Tensin-4 antibody
    • Tns4 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling TNS4/CTEN with Purified ab99887 at 1/100 dilution (0.92 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling TNS4/CTEN with purified ab99887 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).

  • Staining of Human TNS4/CTEN in a Formalin/PFA-fixed paraffin-embedded sections of Human Liver Carcinoma using ab99887 at a dilution of 1/100.

References

ab99887 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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