Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP83] to TNS4/CTEN
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-TNS4/CTEN antibody [SP83]
See all TNS4/CTEN primary antibodies
DescriptionRabbit monoclonal [SP83] to TNS4/CTEN
Tested applicationsSuitable for: IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human TNS4/CTEN aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q8IZW8
- IHC-P: Human Liver Carcinoma, and Human hepatocellular carcinoma tissue; Flow Cyt: HeLa.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com.
This product was previously labelled as TNS4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab99887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min|
|Flow Cyt||Use at an assay dependent concentration.|
FunctionMay be involved in cell migration, cartilage development and in linking signal transduction pathways to the cytoskeleton (By similarity). May promote apoptosis, via its cleavage by caspase-3.
Tissue specificityProstate and placenta. Down regulated in prostate cancer.
Sequence similaritiesContains 1 phosphatase tensin-type domain.
Contains 1 SH2 domain.
modificationsProteolytically cleaved by caspase-3 during apoptosis.
Cellular localizationCell junction > focal adhesion. Cytoplasm > cytoskeleton.
- Information by UniProt
- C terminal tensin like antibody
- C terminal tensin like protein antibody
- C-terminal tensin-like protein antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling TNS4/CTEN with Purified ab99887 at 1/100 dilution (0.92 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling TNS4/CTEN with purified ab99887 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).
Staining of Human TNS4/CTEN in a Formalin/PFA-fixed paraffin-embedded sections of Human Liver Carcinoma using ab99887 at a dilution of 1/100.
ab99887 has not yet been referenced specifically in any publications.