Recombinant Anti-TOB2 antibody [EPR12588] - BSA and Azide free (ab250214)
![Immunoprecipitation - Anti-TOB2 antibody [EPR12588] - BSA and Azide free (ab250214)](https://www.abcam.com/ps/products/250/ab250214/Images/ab250214-2-anti-tob2-antibody-epr12588-bsa-and-azide-free-immunoprecipitation.jpg "Immunoprecipitation - Anti-TOB2 antibody [EPR12588] - BSA and Azide free (ab250214)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12588] to TOB2 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-TOB2 antibody [EPR12588] - BSA and Azide free
See all TOB2 primary antibodies -
Description
Rabbit monoclonal [EPR12588] to TOB2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab250214 is the carrier-free version of ab180514. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab250214 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR12588 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IP |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
|
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 37 kDa. |
Target
-
Function
Anti-proliferative protein inhibits cell cycle progression from the G0/G1 to S phases. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the BTG family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 10766 Human
- Entrez Gene: 57259 Mouse
- Entrez Gene: 315159 Rat
- Omim: 607396 Human
- SwissProt: Q14106 Human
- SwissProt: Q9JM55 Mouse
- Unigene: 474978 Human
- Unigene: 323595 Mouse
-
Alternative names
- KIAA1663 antibody
- Protein Tob2 antibody
- Protein Tob4 antibody
see all
Images
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Immunoprecipitation - Anti-TOB2 antibody [EPR12588] - BSA and Azide free (ab250214)
-
![Western blot - Anti-TOB2 antibody [EPR12588] - BSA and Azide free (ab250214)](https://www.abcam.com/ps/products/250/ab250214/Images/ab250214-398355-anti-tob2-antibody-epr12588-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-TOB2 antibody [EPR12588] - BSA and Azide free (ab250214)All lanes : Anti-TOB2 antibody [EPR12588] - N-terminal (ab180514) at 1/20000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Human skeletal cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDaThis data was developed using ab180514, the same antibody clone in a different buffer formulation.
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![Anti-TOB2 antibody [EPR12588] - BSA and Azide free (ab250214)](https://www.abcam.com/ps/products/250/ab250214/Images/ab250214-3-benefits-of-recombinant-antibodies.png)
Anti-TOB2 antibody [EPR12588] - BSA and Azide free (ab250214)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab250214 has not yet been referenced specifically in any publications.
