Overview

  • Product name

  • Description

    Rabbit polyclonal to Toca-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Toca-1 aa 450-500. The exact sequence is proprietary. (NP_001157945.1)
    Database link: Q5T0N5

  • Positive control

    • WB: Jurkat, HEK-293T and HeLa whole cell lysates. IP: Jurkat whole cell lysate. IHC-P: Human lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab241299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 70 kDa.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles.
  • Sequence similarities

    Belongs to the FNBP1 family.
    Contains 1 FCH domain.
    Contains 1 REM (Hr1) repeat.
    Contains 1 SH3 domain.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1orf39 antibody
    • FBP1L_HUMAN antibody
    • FNBP 1L antibody
    • fnbp1l antibody
    • Formin binding protein 1 like antibody
    • Formin-binding protein 1-like antibody
    • TOCA 1 antibody
    • Toca-1 antibody
    • TOCA1 antibody
    • Transducer of Cdc42 dependent actin assembly 1 antibody
    • Transducer of Cdc42 dependent actin assembly protein 1 antibody
    • Transducer of Cdc42-dependent actin assembly protein 1 antibody
    see all

Images

  • All lanes : Anti-Toca-1 antibody (ab241299) at 0.1 µg/ml

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 70 kDa


    Exposure time: 3 minutes
  • Toca-1 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241299 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different antibody to Toca-1 that recognises an upstream epitope at 1 µg/ml.

    Lane 1: ab241299 IP in Jurkat whole cell lysate.
    Lane 2: Control IgG IP in Jurkat whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

  • Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Toca-1 using ab241299 at 1/1000 dilution in immunohistochemical analysis. DAB staining.

References

ab241299 has not yet been referenced specifically in any publications.

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