Product nameAnti-Toca-1 antibody
See all Toca-1 primary antibodies
DescriptionRabbit polyclonal to Toca-1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Toca-1 (C terminal).
- Mouse brain tissue lysate. Human brain tissue.
Previously labelled as FNBP1L.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab67310 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 70 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionRequired to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles.
Sequence similaritiesBelongs to the FNBP1 family.
Contains 1 FCH domain.
Contains 1 REM (Hr1) repeat.
Contains 1 SH3 domain.
Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle. Cell membrane.
- Information by UniProt
- C1orf39 antibody
- FBP1L_HUMAN antibody
- FNBP 1L antibody
All lanes : Anti-Toca-1 antibody (ab67310) at 1 µg/ml
Lane 1 : Mouse brain tissue lysate with no blocking peptide
Lane 2 : Mouse brain tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 70 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Immunohistochemistry of Toca-1 in human brain tissue using ab67310 at 2.5 µg/ml.
Immunofluorescence of TOCA-1 in Human Brain cells using ab67310 at 20 ug/ml.
ab67310 has not yet been referenced specifically in any publications.