Overview

  • Product name

  • Description

    Rabbit polyclonal to Toca-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Toca-1 (C terminal).

  • Positive control

    • Mouse brain tissue lysate. Human brain tissue.
  • General notes

    Previously labelled as FNBP1L. 

Properties

Applications

Our Abpromise guarantee covers the use of ab67310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 70 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles.
  • Sequence similarities

    Belongs to the FNBP1 family.
    Contains 1 FCH domain.
    Contains 1 REM (Hr1) repeat.
    Contains 1 SH3 domain.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1orf39 antibody
    • FBP1L_HUMAN antibody
    • FNBP 1L antibody
    • fnbp1l antibody
    • Formin binding protein 1 like antibody
    • Formin-binding protein 1-like antibody
    • TOCA 1 antibody
    • Toca-1 antibody
    • TOCA1 antibody
    • Transducer of Cdc42 dependent actin assembly 1 antibody
    • Transducer of Cdc42 dependent actin assembly protein 1 antibody
    • Transducer of Cdc42-dependent actin assembly protein 1 antibody
    see all

Images

  • All lanes : Anti-Toca-1 antibody (ab67310) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate with no blocking peptide
    Lane 2 : Mouse brain tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 70 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry of Toca-1 in human brain tissue using ab67310 at 2.5 µg/ml.

  • Immunofluorescence of TOCA-1 in Human Brain cells using ab67310 at 20 ug/ml.

References

ab67310 has not yet been referenced specifically in any publications.

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