Anti-Tollip antibody (ab37155)

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Western blot - Anti-Tollip antibody (ab37155)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)
  • Immunocytochemistry - Anti-Tollip antibody (ab37155)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)

Key features and details

  • Rabbit polyclonal to Tollip
  • Suitable for: IHC-P, WB, ICC
  • Reacts with: Rat, Human
  • Isotype: IgG

Overview

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab37155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
IHC-P
Human
WB
Rat
Application Abreviews Notes
IHC-P
Use a concentration of 2 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ICC
Use a concentration of 2 µg/ml.
Notes
IHC-P
Use a concentration of 2 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ICC
Use a concentration of 2 µg/ml.

Target

  • Function

    Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.

  • Sequence similarities

    Belongs to the tollip family.
    Contains 1 C2 domain.
    Contains 1 CUE domain.

  • Post-translational
    modifications

    Phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Adapter protein antibody
      • FLJ33531 antibody
      • IL 1RAcPIP antibody
      • IL1RAcPIP antibody
      • TOLIP antibody
      • TOLIP_HUMAN antibody
      • Toll interacting protein antibody
      • Toll-interacting protein antibody
      • tollip antibody
      see all

    Images

    • Western blot - Anti-Tollip antibody (ab37155)
      Western blot - Anti-Tollip antibody (ab37155)
      Lane 1 : Anti-Tollip antibody (ab37155) at 1 µg/ml
      Lane 2 : Anti-Tollip antibody (ab37155) at 2 µg/ml

      All lanes : Rat brain cell lysate

      Predicted band size: 30 kDa
      Observed band size: 30 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)
      Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human brain tissue labeling Tollip with Anti-Tollip antibody (ab37155) at 20μg/ml.
    • Immunocytochemistry - Anti-Tollip antibody (ab37155)
      Immunocytochemistry - Anti-Tollip antibody (ab37155)
      ab37155 at 2 µg/ml staining Tollip in THP-1 cells by ICC. Secondary antibody was biotin conjugated donkey anti-rabbit.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)
      ab37155 staining Tollip in human placenta.
      Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    References (3)

    Publishing research using ab37155? Please let us know so that we can cite the reference in this datasheet.

    ab37155 has been referenced in 3 publications.

    • Zhi H  et al. Tollip Negatively Regulates Vascular Smooth Muscle Cell-Mediated Neointima Formation by Suppressing Akt-Dependent Signaling. J Am Heart Assoc 7:N/A (2018). PubMed: 29887521
    • Iyer RP  et al. Defining the sham environment for post-myocardial infarction studies in mice. Am J Physiol Heart Circ Physiol 311:H822-36 (2016). PubMed: 27521418
    • Vartanian KB  et al. LPS preconditioning redirects TLR signaling following stroke: TRIF-IRF3 plays a seminal role in mediating tolerance to ischemic injury. J Neuroinflammation 8:140 (2011). WB . PubMed: 21999375

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