Anti-Tollip antibody (ab37155)

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Overview

  • Product name

    Anti-Tollip antibody
    See all Tollip primary antibodies

  • Description

    Rabbit polyclonal to Tollip

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ELISA, WB, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to 16 amino acids near to the C terminus of the human Tollip protein.

  • Positive control

    • Rat brain cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab37155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ICC Use a concentration of 2 µg/ml.

Target

Images

  • Western blot - Anti-Tollip antibody (ab37155)
    Western blot - Anti-Tollip antibody (ab37155)
    Lane 1 : Anti-Tollip antibody (ab37155) at 1 µg/ml
    Lane 2 : Anti-Tollip antibody (ab37155) at 2 µg/ml

    All lanes : Rat brain cell lysate

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)
    Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human brain tissue labeling Tollip with Anti-Tollip antibody (ab37155) at 20μg/ml.
  • Immunocytochemistry - Anti-Tollip antibody (ab37155)
    Immunocytochemistry - Anti-Tollip antibody (ab37155)
    ab37155 at 2 µg/ml staining Tollip in THP-1 cells by ICC. Secondary antibody was biotin conjugated donkey anti-rabbit.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)
    ab37155 staining Tollip in human placenta.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References

This product has been referenced in:

  • Zhi H  et al. Tollip Negatively Regulates Vascular Smooth Muscle Cell-Mediated Neointima Formation by Suppressing Akt-Dependent Signaling. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29887521) »
  • Iyer RP  et al. Defining the sham environment for post-myocardial infarction studies in mice. Am J Physiol Heart Circ Physiol 311:H822-36 (2016). Read more (PubMed: 27521418) »
See all 3 Publications for this product

Publishing research using ab37155? Please let us know so that we can cite the reference in this datasheet.

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