Product nameAnti-Tollip antibody
See all Tollip primary antibodies
DescriptionRabbit polyclonal to Tollip
Tested applicationsSuitable for: IHC-P, ELISA, WB, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to 16 amino acids near to the C terminus of the human Tollip protein.
- Rat brain cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab37155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
|ICC||Use a concentration of 2 µg/ml.|
FunctionComponent of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.
Sequence similaritiesBelongs to the tollip family.
Contains 1 C2 domain.
Contains 1 CUE domain.
modificationsPhosphorylated by IRAK1 upon stimulation by IL-1 or microbial products.
- Information by UniProt
- Adapter protein antibody
- FLJ33531 antibody
- IL 1RAcPIP antibody
Lane 1 : Anti-Tollip antibody (ab37155) at 1 µg/ml
Lane 2 : Anti-Tollip antibody (ab37155) at 2 µg/ml
All lanes : Rat brain cell lysate
Predicted band size: 30 kDa
Observed band size: 30 kDa
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human brain tissue labeling Tollip with Anti-Tollip antibody (ab37155) at 20μg/ml.
ab37155 at 2 µg/ml staining Tollip in THP-1 cells by ICC. Secondary antibody was biotin conjugated donkey anti-rabbit.
ab37155 staining Tollip in human placenta.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
This product has been referenced in:
- Zhi H et al. Tollip Negatively Regulates Vascular Smooth Muscle Cell-Mediated Neointima Formation by Suppressing Akt-Dependent Signaling. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29887521) »
- Iyer RP et al. Defining the sham environment for post-myocardial infarction studies in mice. Am J Physiol Heart Circ Physiol 311:H822-36 (2016). Read more (PubMed: 27521418) »