Anti-Tollip antibody (ab37155)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Tollip antibody
See all Tollip primary antibodies -
DescriptionRabbit polyclonal to Tollip
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, ELISA, WB, ICCmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to 16 amino acids near to the C terminus of the human Tollip protein.
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Positive control
- Rat brain cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab37155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 2 µg/ml. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). | |
| ICC | Use a concentration of 2 µg/ml. |
Target
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FunctionComponent of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.
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Sequence similaritiesBelongs to the tollip family.
Contains 1 C2 domain.
Contains 1 CUE domain. -
Post-translational
modificationsPhosphorylated by IRAK1 upon stimulation by IL-1 or microbial products. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 54472 Human
- Entrez Gene: 54473 Mouse
- Entrez Gene: 361677 Rat
- Omim: 606277 Human
- SwissProt: Q9H0E2 Human
- SwissProt: Q9QZ06 Mouse
- SwissProt: A2RUW1 Rat
- Unigene: 368527 Human
see all -
Alternative names
- Adapter protein antibody
- FLJ33531 antibody
- IL 1RAcPIP antibody
see all
Images
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Western blot - Anti-Tollip antibody (ab37155)Lane 1 : Anti-Tollip antibody (ab37155) at 1 µg/ml
Lane 2 : Anti-Tollip antibody (ab37155) at 2 µg/ml
All lanes : Rat brain cell lysate
Predicted band size: 30 kDa
Observed band size: 30 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human brain tissue labeling Tollip with Anti-Tollip antibody (ab37155) at 20μg/ml. -
Immunocytochemistry - Anti-Tollip antibody (ab37155)ab37155 at 2 µg/ml staining Tollip in THP-1 cells by ICC. Secondary antibody was biotin conjugated donkey anti-rabbit. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tollip antibody (ab37155)ab37155 staining Tollip in human placenta.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Vartanian KB et al. LPS preconditioning redirects TLR signaling following stroke: TRIF-IRF3 plays a seminal role in mediating tolerance to ischemic injury. J Neuroinflammation 8:140 (2011). WB . Read more (PubMed: 21999375) »
