Key features and details
- Rabbit polyclonal to Tollip
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Tollip antibody
See all Tollip primary antibodies
DescriptionRabbit polyclonal to Tollip
Tested applicationsSuitable for: ICC, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
A 16 amino acid peptide from near the center of human Tollip
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 2 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
FunctionComponent of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.
Sequence similaritiesBelongs to the tollip family.
Contains 1 C2 domain.
Contains 1 CUE domain.
modificationsPhosphorylated by IRAK1 upon stimulation by IL-1 or microbial products.
- Information by UniProt
- Adapter protein antibody
- FLJ33531 antibody
- IL 1RAcPIP antibody
ab62578 at 2µg/ml staining Tollip in THP-1 cells by Immunocytochemistry.
Lane 1 : Anti-Tollip antibody (ab62578) at 0.5 µg/ml
Lane 2 : Anti-Tollip antibody (ab62578) at 1 µg/ml
Lane 3 : Anti-Tollip antibody (ab62578) at 2 µg/ml
All lanes : rat brain cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 30 kDa
Observed band size: 30 kDa
ab62578 has been referenced in 1 publication.
- Sugi Y et al. Post-Transcriptional Regulation of Toll-Interacting Protein in the Intestinal Epithelium. PLoS One 11:e0164858 (2016). WB ; Mouse . PubMed: 27741296