Anti-Tollip antibody (ab62578)
Key features and details
- Rabbit polyclonal to Tollip
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Tollip antibody
See all Tollip primary antibodies -
Description
Rabbit polyclonal to Tollip -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
A 16 amino acid peptide from near the center of human Tollip
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab62578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use a concentration of 2 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. -
Sequence similarities
Belongs to the tollip family.
Contains 1 C2 domain.
Contains 1 CUE domain. -
Post-translational
modificationsPhosphorylated by IRAK1 upon stimulation by IL-1 or microbial products. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 54472 Human
- Entrez Gene: 54473 Mouse
- Entrez Gene: 361677 Rat
- Omim: 606277 Human
- SwissProt: Q9H0E2 Human
- SwissProt: Q9QZ06 Mouse
- SwissProt: A2RUW1 Rat
- Unigene: 368527 Human
see all -
Alternative names
- Adapter protein antibody
- FLJ33531 antibody
- IL 1RAcPIP antibody
see all
Images
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Immunocytochemistry - Anti-Tollip antibody (ab62578)ab62578 at 2µg/ml staining Tollip in THP-1 cells by Immunocytochemistry.
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Western blot - Anti-Tollip antibody (ab62578)Lane 1 : Anti-Tollip antibody (ab62578) at 0.5 µg/ml
Lane 2 : Anti-Tollip antibody (ab62578) at 1 µg/ml
Lane 3 : Anti-Tollip antibody (ab62578) at 2 µg/ml
All lanes : rat brain cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Protocols
Datasheets and documents
References (1)
ab62578 has been referenced in 1 publication.
- Sugi Y et al. Post-Transcriptional Regulation of Toll-Interacting Protein in the Intestinal Epithelium. PLoS One 11:e0164858 (2016). WB ; Mouse . PubMed: 27741296
