Overview

  • Product name
    Anti-Tollip antibody
    See all Tollip primary antibodies
  • Description
    Rabbit polyclonal to Tollip
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, ICC, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid peptide from near the center of human Tollip

  • Positive control
    • Rat brain cell lysate and THP-1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
ICC Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

Target

Images

  • Immunocytochemistry - Anti-Tollip antibody (ab62578)
    Immunocytochemistry - Anti-Tollip antibody (ab62578)
    ab62578 at 2µg/ml staining Tollip in THP-1 cells by Immunocytochemistry.
  • Western blot - Anti-Tollip antibody (ab62578)
    Western blot - Anti-Tollip antibody (ab62578)
    Lane 1 : Anti-Tollip antibody (ab62578) at 0.5 µg/ml
    Lane 2 : Anti-Tollip antibody (ab62578) at 1 µg/ml
    Lane 3 : Anti-Tollip antibody (ab62578) at 2 µg/ml

    All lanes : rat brain cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

References

This product has been referenced in:
  • Sugi Y  et al. Post-Transcriptional Regulation of Toll-Interacting Protein in the Intestinal Epithelium. PLoS One 11:e0164858 (2016). WB ; Mouse . Read more (PubMed: 27741296) »

See 1 Publication for this product

Publishing research using ab62578? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-Tollip antibody

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Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycine)
Sample
Rat Cell lysate - whole cell (rat brain)
Specification
rat brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted Aug 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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