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  1. Link

    tollip-antibody-ab62578.pdf

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Immunology Immunoglobulins Receptors
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Anti-Tollip antibody (ab62578)

  • Datasheet
Reviews (1) Submit a question References (1)

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Immunocytochemistry - Anti-Tollip antibody (ab62578)
  • Western blot - Anti-Tollip antibody (ab62578)

Key features and details

  • Rabbit polyclonal to Tollip
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Tollip antibody
    See all Tollip primary antibodies
  • Description

    Rabbit polyclonal to Tollip
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 16 amino acid peptide from near the center of human Tollip

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Receptors
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Tollip protein (ab172165)

Applications

Our Abpromise guarantee covers the use of ab62578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

Target

  • Function

    Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity.
  • Sequence similarities

    Belongs to the tollip family.
    Contains 1 C2 domain.
    Contains 1 CUE domain.
  • Post-translational
    modifications

    Phosphorylated by IRAK1 upon stimulation by IL-1 or microbial products.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q9H0E2 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 54472 Human
    • Entrez Gene: 54473 Mouse
    • Entrez Gene: 361677 Rat
    • Omim: 606277 Human
    • SwissProt: Q9H0E2 Human
    • SwissProt: Q9QZ06 Mouse
    • SwissProt: A2RUW1 Rat
    • Unigene: 368527 Human
    • Unigene: 103551 Mouse
    • Unigene: 137040 Rat
    see all
  • Alternative names

    • Adapter protein antibody
    • FLJ33531 antibody
    • IL 1RAcPIP antibody
    • IL1RAcPIP antibody
    • TOLIP antibody
    • TOLIP_HUMAN antibody
    • Toll interacting protein antibody
    • Toll-interacting protein antibody
    • tollip antibody
    see all

Images

  • Immunocytochemistry - Anti-Tollip antibody (ab62578)
    Immunocytochemistry - Anti-Tollip antibody (ab62578)
    ab62578 at 2µg/ml staining Tollip in THP-1 cells by Immunocytochemistry.
  • Western blot - Anti-Tollip antibody (ab62578)
    Western blot - Anti-Tollip antibody (ab62578)
    Lane 1 : Anti-Tollip antibody (ab62578) at 0.5 µg/ml
    Lane 2 : Anti-Tollip antibody (ab62578) at 1 µg/ml
    Lane 3 : Anti-Tollip antibody (ab62578) at 2 µg/ml

    All lanes : rat brain cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab62578? Please let us know so that we can cite the reference in this datasheet.

    ab62578 has been referenced in 1 publication.

    • Sugi Y  et al. Post-Transcriptional Regulation of Toll-Interacting Protein in the Intestinal Epithelium. PLoS One 11:e0164858 (2016). WB ; Mouse . PubMed: 27741296

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Tollip antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (4-20% Tris-Glycine)
    Sample
    Rat Cell lysate - whole cell (rat brain)
    Specification
    rat brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 12 2013

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