Anti-Tollip antibody (ab62578)

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Overview

  • Product name

    Anti-Tollip antibody
    See all Tollip primary antibodies

  • Description

    Rabbit polyclonal to Tollip

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    A 16 amino acid peptide from near the center of human Tollip

  • Positive control

    • Rat brain cell lysate and THP-1 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
ICC Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

Target

Images

  • Immunocytochemistry - Anti-Tollip antibody (ab62578)
    Immunocytochemistry - Anti-Tollip antibody (ab62578)
    ab62578 at 2µg/ml staining Tollip in THP-1 cells by Immunocytochemistry.
  • Western blot - Anti-Tollip antibody (ab62578)
    Western blot - Anti-Tollip antibody (ab62578)
    Lane 1 : Anti-Tollip antibody (ab62578) at 0.5 µg/ml
    Lane 2 : Anti-Tollip antibody (ab62578) at 1 µg/ml
    Lane 3 : Anti-Tollip antibody (ab62578) at 2 µg/ml

    All lanes : rat brain cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 30 kDa
    Observed band size: 30 kDa

References

This product has been referenced in:

  • Sugi Y  et al. Post-Transcriptional Regulation of Toll-Interacting Protein in the Intestinal Epithelium. PLoS One 11:e0164858 (2016). WB ; Mouse . Read more (PubMed: 27741296) »
See 1 Publication for this product

Publishing research using ab62578? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-Tollip antibody

 Poor
Abreviews
abreview image
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycine)
Sample
Rat Cell lysate - whole cell (rat brain)
Specification
rat brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

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Submitted Aug 12 2013

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