Key features and details
- Rabbit polyclonal to TOM1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TOM1 antibody
See all TOM1 primary antibodies
DescriptionRabbit polyclonal to TOM1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human TOM1 aa 204-380.
LSGDTPIAPTPEQIGKLRSELEMVSGNVRVMSEMLTELVPTQAEPADLEL LQELNRTCRAMQQRVLELIPQIANEQLTEELLIVNDNLNNVFLRHERFER FRTGQTTKAPSEAEPAADLIDMGPDPAATGNLSSQLAGMNLGSSSVRAGL QSLEASGRLEDEFDMFALTRGSSLADQ
Database link: O60784
- WB: RT-4, EFO-21 and A431 cell lysate; human liver and tonsil tissue lysate. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab219972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 54 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
RelevanceFunction: May be involved in intracellular trafficking. Probable association with membranes. Tissue specificity: Widely expressed. Highly expressed in skeletal muscle, heart, placenta and liver. Similarity: Belongs to the TOM1 family. Contains 1 GAT domain. Contains 1 VHS domain.
Cellular localizationCytoplasm. Membrane; Peripheral membrane protein
- FLJ33404 antibody
- OTTHUMP00000028777 antibody
- Target of myb 1 antibody
All lanes : Anti-TOM1 antibody (ab219972) at 1/250 dilution
Lane 1 : RT-4 cell line lysate
Lane 2 : EFO-21 cell line lysate
Lane 3 : A431 cell line lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 54 kDa
Immunofluorescent analysis of paraformaldehyde-fixed, Triton X-100 permeabilized U-2 OS cells labeling TOM1 with ab219972 at 4 μg/mL dilution (green).
ab219972 has not yet been referenced specifically in any publications.