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    tom1-antibody-ab99356.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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Anti-TOM1 antibody (ab99356)

  • Datasheet
  • SDS
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Western blot - Anti-TOM1 antibody (ab99356)
  • Immunoprecipitation - Anti-TOM1 antibody (ab99356)

Key features and details

  • Rabbit polyclonal to TOM1
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human TOM1 protein (ab160775)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TOM1 antibody
    See all TOM1 primary antibodies
  • Description

    Rabbit polyclonal to TOM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 450-492 of Human TOM1 (NP_005479.1).

  • Positive control

    • HeLa and 293T whole cell lysates
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab99356 was affinity purified using an epitope specific to TOM1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • HeLa membrane extract lysate (ab29547)
  • Recombinant Protein

    • Recombinant Human TOM1 protein (ab160775)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab99356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP
Use at 2-5 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP
Use at 2-5 µg/mg of lysate.

Target

  • Relevance

    Function: May be involved in intracellular trafficking. Probable association with membranes. Tissue specificity: Widely expressed. Highly expressed in skeletal muscle, heart, placenta and liver. Similarity: Belongs to the TOM1 family. Contains 1 GAT domain. Contains 1 VHS domain.
  • Cellular localization

    Cytoplasm. Membrane; Peripheral membrane protein
  • Database links

    • Entrez Gene: 739845 Chimpanzee
    • Entrez Gene: 10043 Human
    • Omim: 604700 Human
    • SwissProt: O60784 Human
    • Unigene: 474705 Human
    • Alternative names

      • FLJ33404 antibody
      • OTTHUMP00000028777 antibody
      • Target of myb 1 antibody
      • Target of Myb protein 1 antibody
      • Target of myb1 antibody
      • TOM 1 antibody
      • TOM1 antibody
      • TOM1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-TOM1 antibody (ab99356)
      Western blot - Anti-TOM1 antibody (ab99356)
      All lanes : Anti-TOM1 antibody (ab99356) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 54 kDa


      Exposure time: 30 seconds
    • Immunoprecipitation - Anti-TOM1 antibody (ab99356)
      Immunoprecipitation - Anti-TOM1 antibody (ab99356)
      ab99356, at 1 µg/ml, detecting TOM1 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure.
      For immunoprecipitation, ab99356 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
      Lane 1; IP using ab99355 (a rabbit anti-TOM1 antibody which recognizes an upstream epitope).
      Lane 2; IP using ab99356.
      Lane 3; IP using control IgG.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab99356? Please let us know so that we can cite the reference in this datasheet.

    ab99356 has been referenced in 3 publications.

    • Ryan TA  et al. Tollip coordinates Parkin-dependent trafficking of mitochondrial-derived vesicles. EMBO J 39:e102539 (2020). PubMed: 32311122
    • Hu S  et al. Structure of Myosin VI/Tom1 complex reveals a cargo recognition mode of Myosin VI for tethering. Nat Commun 10:3459 (2019). PubMed: 31371777
    • Boal F  et al. TOM1 is a PI5P effector involved in the regulation of endosomal maturation. J Cell Sci 128:815-27 (2015). PubMed: 25588840

    Customer reviews and Q&As

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