Anti-TOM1 antibody (ab99356)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-TOM1 antibody
See all TOM1 primary antibodies -
Description
Rabbit polyclonal to TOM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
Synthetic peptide corresponding to a region within amino acids 450-492 of Human TOM1 (NP_005479.1).
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Positive control
- HeLa and 293T whole cell lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab99356 was affinity purified using an epitope specific to TOM1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab99356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 54 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. |
Target
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Relevance
Function: May be involved in intracellular trafficking. Probable association with membranes. Tissue specificity: Widely expressed. Highly expressed in skeletal muscle, heart, placenta and liver. Similarity: Belongs to the TOM1 family. Contains 1 GAT domain. Contains 1 VHS domain. -
Cellular localization
Cytoplasm. Membrane; Peripheral membrane protein -
Database links
- Entrez Gene: 739845 Chimpanzee
- Entrez Gene: 10043 Human
- Omim: 604700 Human
- SwissProt: O60784 Human
- Unigene: 474705 Human
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Alternative names
- FLJ33404 antibody
- OTTHUMP00000028777 antibody
- Target of myb 1 antibody
see all
Images
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Western blot - Anti-TOM1 antibody (ab99356)All lanes : Anti-TOM1 antibody (ab99356) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-TOM1 antibody (ab99356)ab99356, at 1 µg/ml, detecting TOM1 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure.
For immunoprecipitation, ab99356 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
Lane 1; IP using ab99355 (a rabbit anti-TOM1 antibody which recognizes an upstream epitope).
Lane 2; IP using ab99356.
Lane 3; IP using control IgG.
Datasheets and documents
References
This product has been referenced in:
- Hu S et al. Structure of Myosin VI/Tom1 complex reveals a cargo recognition mode of Myosin VI for tethering. Nat Commun 10:3459 (2019). Read more (PubMed: 31371777) »
- Boal F et al. TOM1 is a PI5P effector involved in the regulation of endosomal maturation. J Cell Sci 128:815-27 (2015). Read more (PubMed: 25588840) »
