Product nameAnti-TOM1 antibody
See all TOM1 primary antibodies
DescriptionRabbit polyclonal to TOM1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide corresponding to a region within amino acids 450-492 of Human TOM1 (NP_005479.1).
- HeLa and 293T whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab99356 was affinity purified using an epitope specific to TOM1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab99356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 54 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceFunction: May be involved in intracellular trafficking. Probable association with membranes. Tissue specificity: Widely expressed. Highly expressed in skeletal muscle, heart, placenta and liver. Similarity: Belongs to the TOM1 family. Contains 1 GAT domain. Contains 1 VHS domain.
Cellular localizationCytoplasm. Membrane; Peripheral membrane protein
- FLJ33404 antibody
- OTTHUMP00000028777 antibody
- Target of myb 1 antibody
All lanes : Anti-TOM1 antibody (ab99356) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Exposure time: 30 seconds
ab99356, at 1 µg/ml, detecting TOM1 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure.
For immunoprecipitation, ab99356 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
Lane 1; IP using ab99355 (a rabbit anti-TOM1 antibody which recognizes an upstream epitope).
Lane 2; IP using ab99356.
Lane 3; IP using control IgG.
This product has been referenced in:
- Hu S et al. Structure of Myosin VI/Tom1 complex reveals a cargo recognition mode of Myosin VI for tethering. Nat Commun 10:3459 (2019). Read more (PubMed: 31371777) »
- Boal F et al. TOM1 is a PI5P effector involved in the regulation of endosomal maturation. J Cell Sci 128:815-27 (2015). Read more (PubMed: 25588840) »