Recombinant Anti-TOM1 antibody [EPR11988(B)] (ab170866)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11988(B)] to TOM1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TOM1 antibody [EPR11988(B)]
See all TOM1 primary antibodies -
Description
Rabbit monoclonal [EPR11988(B)] to TOM1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human TOM1 (Cysteine residue). The exact sequence is proprietary.
Database link: O60784 -
Positive control
- HepG2, JAR, HeLa and K562 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11988(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 54 kDa. |
Target
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Relevance
Function: May be involved in intracellular trafficking. Probable association with membranes. Tissue specificity: Widely expressed. Highly expressed in skeletal muscle, heart, placenta and liver. Similarity: Belongs to the TOM1 family. Contains 1 GAT domain. Contains 1 VHS domain. -
Cellular localization
Cytoplasm. Membrane; Peripheral membrane protein -
Database links
- Entrez Gene: 10043 Human
- Entrez Gene: 21968 Mouse
- Entrez Gene: 361370 Rat
- Omim: 604700 Human
- SwissProt: O60784 Human
- SwissProt: O88746 Mouse
- SwissProt: Q5XI21 Rat
- Unigene: 474705 Human
see all -
Alternative names
- FLJ33404 antibody
- OTTHUMP00000028777 antibody
- Target of myb 1 antibody
see all
Images
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All lanes : Anti-TOM1 antibody [EPR11988(B)] (ab170866) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TOM1 knockout HAP1 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 54 kDaLanes 1 - 4: Merged signal (red and green). Green - ab170866 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab170866 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in TOM1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TOM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab170866 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-TOM1 antibody [EPR11988(B)] (ab170866) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : JAR cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : K562 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab170866 has not yet been referenced specifically in any publications.