Recombinant Anti-TOM1 antibody [EPR11989(B)] (ab170928)
![Flow Cytometry - Anti-TOM1 antibody [EPR11989(B)] (ab170928)](https://www.abcam.com/ps/products/170/ab170928/Images/ab170928-170814-anti-tom1-antibody-epr11989b-flow-cytometry.jpg "Flow Cytometry - Anti-TOM1 antibody [EPR11989(B)] (ab170928)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11989(B)] to TOM1
- Suitable for: WB, IHC-P, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-TOM1 antibody [EPR11989(B)]
See all TOM1 primary antibodies -
Description
Rabbit monoclonal [EPR11989(B)] to TOM1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TOM1 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O60784 -
Positive control
- HepG2, HeLa, K562 and JAR cell lysates; Human liver and stomach tissues; K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11989(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 54 kDa.
|
|
| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 54 kDa. |
|
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Relevance
Function: May be involved in intracellular trafficking. Probable association with membranes. Tissue specificity: Widely expressed. Highly expressed in skeletal muscle, heart, placenta and liver. Similarity: Belongs to the TOM1 family. Contains 1 GAT domain. Contains 1 VHS domain. -
Cellular localization
Cytoplasm. Membrane; Peripheral membrane protein -
Database links
- Entrez Gene: 10043 Human
- Entrez Gene: 21968 Mouse
- Entrez Gene: 361370 Rat
- Omim: 604700 Human
- SwissProt: O60784 Human
- SwissProt: O88746 Mouse
- SwissProt: Q5XI21 Rat
- Unigene: 474705 Human
see all -
Alternative names
- FLJ33404 antibody
- OTTHUMP00000028777 antibody
- Target of myb 1 antibody
see all
Images
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Flow Cytometry - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
Flow cytometric analysis of permeabilized K562 cells labeling TOM1 with ab170928 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
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![Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)](https://www.abcam.com/ps/products/170/ab170928/Images/ab170928-365779-anti-tom1-antibody-epr11989b-western-blot-knockout-hap1-tom1-hepg2-hela-human-lysates.jpg)
Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)All lanes : Anti-TOM1 antibody [EPR11989(B)] (ab170928) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : TOM1 knockout HAP1 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 54 kDaLanes 1 - 4: Merged signal (red and green). Green - ab170928 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab170928 was shown to specifically react with in wild-type HAP1 cells as signal was lost in TOM1 knockout cells. Wild-type and TOM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab170928 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)](https://www.abcam.com/ps/products/170/ab170928/Images/ab170928-170813-anti-tom1-antibody-epr11989b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling TOM1 with ab170928 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)](https://www.abcam.com/ps/products/170/ab170928/Images/ab170928-170812-anti-tom1-antibody-epr11989b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOM1 with ab170928 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)](https://www.abcam.com/ps/products/170/ab170928/Images/ab170928-170811-anti-tom1-antibody-epr11989b-western-blot.jpg)
Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)All lanes : Anti-TOM1 antibody [EPR11989(B)] (ab170928) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 54 kDa -
![Anti-TOM1 antibody [EPR11989(B)] (ab170928)](https://www.abcam.com/ps/products/170/ab170928/Images/ab170928-6-benefits-of-recombinant-antibodies.png)
Anti-TOM1 antibody [EPR11989(B)] (ab170928)
Protocols
Datasheets and documents
References (1)
ab170928 has been referenced in 1 publication.
- Lobingier BT et al. An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells. Cell 169:350-360.e12 (2017). PubMed: 28388416
