Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-TOM1 antibody [EPR11989(B)] (ab170928)

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Overview

  • Product name

    Anti-TOM1 antibody [EPR11989(B)]
    See all TOM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11989(B)] to TOM1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TOM1 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O60784

  • Positive control

    • HepG2, HeLa, K562 and JAR cell lysates; Human liver and stomach tissues; K562 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11989(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 54 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC or IP.

    • Target

    • Relevance

      Function: May be involved in intracellular trafficking. Probable association with membranes. Tissue specificity: Widely expressed. Highly expressed in skeletal muscle, heart, placenta and liver. Similarity: Belongs to the TOM1 family. Contains 1 GAT domain. Contains 1 VHS domain.

    • Cellular localization

      Cytoplasm. Membrane; Peripheral membrane protein

    • Database links

      see all

    • Alternative names

      • FLJ33404 antibody
      • OTTHUMP00000028777 antibody
      • Target of myb 1 antibody
      • Target of Myb protein 1 antibody
      • Target of myb1 antibody
      • TOM 1 antibody
      • TOM1 antibody
      • TOM1_HUMAN antibody
      see all

    Images

    • Flow Cytometry - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      Flow Cytometry - Anti-TOM1 antibody [EPR11989(B)] (ab170928)

      Flow cytometric analysis of permeabilized K562 cells labeling TOM1 with ab170928 at 1/100 dilution (red)  compared to a rabbit IgG negative control (green).

    • Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      All lanes : Anti-TOM1 antibody [EPR11989(B)] (ab170928) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : TOM1 knockout HAP1 whole cell lysate
      Lane 3 : HepG2 whole cell lysate
      Lane 4 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 54 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab170928 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab170928 was shown to specifically react with in wild-type HAP1 cells as signal was lost in TOM1 knockout cells. Wild-type and TOM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab170928 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)

      Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling TOM1 with ab170928 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOM1 with ab170928 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      All lanes : Anti-TOM1 antibody [EPR11989(B)] (ab170928) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 54 kDa

    References

    This product has been referenced in:

    • Lobingier BT  et al. An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells. Cell 169:350-360.e12 (2017). Read more (PubMed: 28388416) »
    See 1 Publication for this product

    Publishing research using ab170928? Please let us know so that we can cite the reference in this datasheet.

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