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    tom1-antibody-epr11989b-ab170928.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TOM1 antibody [EPR11989(B)] (ab170928)

  • Datasheet
Submit a review Submit a question References (1)

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Flow Cytometry - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
  • Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
  • Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
  • Anti-TOM1 antibody [EPR11989(B)] (ab170928)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11989(B)] to TOM1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-TOM1 antibody [EPR11989(B)]
    See all TOM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11989(B)] to TOM1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TOM1 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O60784

  • Positive control

    • HepG2, HeLa, K562 and JAR cell lysates; Human liver and stomach tissues; K562 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11989(B)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Alternative Versions

    • Anti-TOM1 antibody [EPR11989(B)] - BSA and Azide free (ab249599)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • HeLa whole cell lysate (ab29545)
    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human TOM1 protein (ab160775)
  • Related Products

    • Recombinant Human TOM1 protein (ab160775)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Human
IHC-P
Human
WB
Human
All applications
Rat
Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 54 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 54 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Relevance

      Function: May be involved in intracellular trafficking. Probable association with membranes. Tissue specificity: Widely expressed. Highly expressed in skeletal muscle, heart, placenta and liver. Similarity: Belongs to the TOM1 family. Contains 1 GAT domain. Contains 1 VHS domain.
    • Cellular localization

      Cytoplasm. Membrane; Peripheral membrane protein
    • Database links

      • Entrez Gene: 10043 Human
      • Entrez Gene: 21968 Mouse
      • Entrez Gene: 361370 Rat
      • Omim: 604700 Human
      • SwissProt: O60784 Human
      • SwissProt: O88746 Mouse
      • SwissProt: Q5XI21 Rat
      • Unigene: 474705 Human
      • Unigene: 1967 Mouse
      • Unigene: 290868 Mouse
      see all
    • Alternative names

      • FLJ33404 antibody
      • OTTHUMP00000028777 antibody
      • Target of myb 1 antibody
      • Target of Myb protein 1 antibody
      • Target of myb1 antibody
      • TOM 1 antibody
      • TOM1 antibody
      • TOM1_HUMAN antibody
      see all

    Images

    • Flow Cytometry - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      Flow Cytometry - Anti-TOM1 antibody [EPR11989(B)] (ab170928)

      Flow cytometric analysis of permeabilized K562 cells labeling TOM1 with ab170928 at 1/100 dilution (red)  compared to a rabbit IgG negative control (green).

    • Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      All lanes : Anti-TOM1 antibody [EPR11989(B)] (ab170928) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : TOM1 knockout HAP1 whole cell lysate
      Lane 3 : HepG2 whole cell lysate
      Lane 4 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 54 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab170928 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab170928 was shown to specifically react with in wild-type HAP1 cells as signal was lost in TOM1 knockout cells. Wild-type and TOM1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab170928 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)

      Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling TOM1 with ab170928 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TOM1 antibody [EPR11989(B)] (ab170928)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TOM1 with ab170928 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      Western blot - Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      All lanes : Anti-TOM1 antibody [EPR11989(B)] (ab170928) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 54 kDa

    • Anti-TOM1 antibody [EPR11989(B)] (ab170928)
      Anti-TOM1 antibody [EPR11989(B)] (ab170928)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab170928? Please let us know so that we can cite the reference in this datasheet.

    ab170928 has been referenced in 1 publication.

    • Lobingier BT  et al. An Approach to Spatiotemporally Resolve Protein Interaction Networks in Living Cells. Cell 169:350-360.e12 (2017). PubMed: 28388416

    Customer reviews and Q&As

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