Key features and details
- Rabbit polyclonal to TOM1L2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-TOM1L2 antibody
See all TOM1L2 primary antibodies
DescriptionRabbit polyclonal to TOM1L2
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human heart muscle tissue; RT4, U251 MG, Human plasma, liver and tonsil lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121716 in the following tested applications.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionProbable role in protein transport. May regulate growth factor-induced mitogenic signaling.
Tissue specificityUbiquitously expressed with higher expression in heart and skeletal muscle.
Sequence similaritiesBelongs to the TOM1 family.
Contains 1 GAT domain.
Contains 1 VHS domain.
DomainThe GAT domain mediates interaction with TOLLIP.
- Information by UniProt
- FLJ32746 antibody
- Srebf1 antibody
- Target of Myb-like protein 2 antibody
Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nuclei but not nucleoli. Recommended concentration of ab121716 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121716 at 1/250 dilution staining TOM1L2 in Paraffin-embedded Human heart muscle tissue by Immunohistochemistry.
All lanes : Anti-TOM1L2 antibody (ab121716) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver tissue lysate
Lane 5 : Human Tonsil tissue lysate
Developed using the ECL technique.
Immunohistochemical staining of Human heart muscle shows moderate cytoplasmic positivity in myocytes using ab121716
ab121716 has not yet been referenced specifically in any publications.