Key features and details
- Rabbit polyclonal to TOM70
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TOM70 antibody
See all TOM70 primary antibodies
DescriptionRabbit polyclonal to TOM70
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- IHC-P: Human pancreas, cerebral cortex, small intestine and prostate tissues. ICC/IF: U-2 OS cells. WB: PC-3 cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab251925 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 67 kDa.|
FunctionReceptor that accelerates the import of all mitochondrial precursor proteins.
Sequence similaritiesBelongs to the Tom70 family.
Contains 10 TPR repeats.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- FLJ9047 antibody
- Mitochondrial import receptor subunit TOM70 antibody
- Mitochondrial precursor proteins import receptor antibody
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for TOM70 (green) using ab251925 at 4 µg/ml in ICC/IF.
Anti-TOM70 antibody (ab251925) at 0.04 µg/ml + PC-3 (Human prostate adenocarcinoma) cell lysate
Predicted band size: 67 kDa
Western blot analysis of PC-3 (Human prostate adenocarcinoma) cell lysate labeling TOM70 with ab251925.
Paraffin-embedded human small intestine tissue stained for TOM70 using ab251925 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate tissue stained for TOM70 using ab251925 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebral cortex tissue stained for TOM70 using ab251925 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreas tissue stained for TOM70 using ab251925 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab251925 has not yet been referenced specifically in any publications.