Anti-TOM70 antibody (ab89624)
Key features and details
- Mouse polyclonal to TOM70
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TOM70 antibody
See all TOM70 primary antibodies -
Description
Mouse polyclonal to TOM70 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human TOM70. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_055635).
-
Positive control
- Human liver tissue lysate, HepG2 whole cell lysate (ab7900) and TOM70 transfected 293T cell lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa.
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. |
Target
-
Function
Receptor that accelerates the import of all mitochondrial precursor proteins. -
Sequence similarities
Belongs to the Tom70 family.
Contains 10 TPR repeats. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
-
Database links
- Entrez Gene: 9868 Human
- Omim: 606081 Human
- SwissProt: O94826 Human
- Unigene: 227253 Human
-
Alternative names
- FLJ9047 antibody
- Mitochondrial import receptor subunit TOM70 antibody
- Mitochondrial precursor proteins import receptor antibody
see all
Images
-
Anti-TOM70 antibody (ab89624) at 1 µg/ml + human liver tissue lysate at 50 µg
Predicted band size: 67 kDa
Observed band size: 67 kDa -
Anti-TOM70 antibody (ab89624) at 1 µg/ml + HepG2 cell lysate at 50 µg
Predicted band size: 67 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
All lanes : Anti-TOM70 antibody (ab89624) at 1 µg/ml
Lane 1 : TOMM70A transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 67 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
Datasheet download
References (4)
ab89624 has been referenced in 4 publications.
- Richter M et al. The Recombinant Fragment of Human ?-Casein Induces Cell Death by Targeting the Proteins of Mitochondrial Import in Breast Cancer Cells. Cancers (Basel) 12:N/A (2020). PubMed: 32486420
- Reyes A et al. RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases. PLoS Genet 16:e1008923 (2020). PubMed: 32735630
- Wohlfromm F et al. Interplay Between Mitophagy and Apoptosis Defines a Cell Fate Upon Co-treatment of Breast Cancer Cells With a Recombinant Fragment of Human ?-Casein and Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand. Front Cell Dev Biol 8:617762 (2020). PubMed: 33537307
- Rakovic A et al. Phosphatase and tensin homolog (PTEN)-induced putative kinase 1 (PINK1)-dependent ubiquitination of endogenous Parkin attenuates mitophagy: study in human primary fibroblasts and induced pluripotent stem cell-derived neurons. J Biol Chem 288:2223-37 (2013). WB ; Human . PubMed: 23212910