Overview

  • Product name

  • Description

    Mouse polyclonal to TOM70
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human TOM70. (NP_055635).

  • Positive control

    • Human liver tissue lysate, HepG2 whole cell lysate (ab7900) and TOM70 transfected 293T cell lysate
  • General notes

    Protein previously labeled as TOMM70A.

Properties

Applications

Our Abpromise guarantee covers the use of ab89624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa.

Target

  • Function

    Receptor that accelerates the import of all mitochondrial precursor proteins.
  • Sequence similarities

    Belongs to the Tom70 family.
    Contains 10 TPR repeats.
  • Cellular localization

    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ9047 antibody
    • Mitochondrial import receptor subunit TOM70 antibody
    • Mitochondrial precursor proteins import receptor antibody
    • TOM70 antibody
    • TOM70_HUMAN antibody
    • Tomm70a antibody
    • Translocase of outer membrane 70 kDa subunit antibody
    • Translocase of outer membrane TOM70 antibody
    • Translocase of outer mitochondrial membrane 70 homolog A antibody
    see all

Images

  • Anti-TOM70 antibody (ab89624) at 1 µg/ml + human liver tissue lysate at 50 µg

    Predicted band size: 67 kDa
    Observed band size: 67 kDa

  • Anti-TOM70 antibody (ab89624) at 1 µg/ml + HepG2 cell lysate at 50 µg

    Predicted band size: 67 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-TOM70 antibody (ab89624) at 1 µg/ml

    Lane 1 : TOMM70A transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 67 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Rakovic A  et al. Phosphatase and tensin homolog (PTEN)-induced putative kinase 1 (PINK1)-dependent ubiquitination of endogenous Parkin attenuates mitophagy: study in human primary fibroblasts and induced pluripotent stem cell-derived neurons. J Biol Chem 288:2223-37 (2013). WB ; Human . Read more (PubMed: 23212910) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (breast cancer cells)
Total protein in input
2000 µg
Treatment
n/a
Immuno-precipitation step
Protein A
Specification
breast cancer cells

Max Richter

Verified customer

Submitted Nov 14 2017

Answer

Thank you for contacting us.

Looking at the sequence alignment between the Human TOMM70A and the yeast TOMM70A (see attachment), I'm afraid that none of the anti-TOMM70A we have in our catalogseems to besuitable for yeast.

I am sorry I cannot be more helpfulon this occasion. Please do not hesitate to contact us if you need any more advice or information.

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