Recombinant Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) - BSA and Azide free (ab283339)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [EPR15581-39] to TOMM20 - Chimeric – BSA and Azide free
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) - BSA and Azide free
See all TOMM20 primary antibodies -
Description
Mouse monoclonal [EPR15581-39] to TOMM20 - Chimeric – BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, NIH/3T3, C6 whole cell lysates. IHC-P: Human liver, Mouse kidney, Rat kidney tissues. IHC-Fr: Mouse and Rat small intestine. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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General notes
Mitochondrial Marker
ab283339 is the carrier-free version of ab283317.
This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab186734). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15581-39 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] (ab309083)
- Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (ab309166)
- Alexa Fluor® 647 Anti-TOMM20 antibody [EPR15581-39] - Rat IgG2a (Chimeric) (ab309167)
- Alexa Fluor® 488 Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) (ab314475)
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab283339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
Use at an assay dependent concentration.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Use at an assay dependent concentration. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. -
Sequence similarities
Belongs to the Tom20 family. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 9804 Human
- Entrez Gene: 67952 Mouse
- Entrez Gene: 266601 Rat
- Omim: 601848 Human
- SwissProt: Q15388 Human
- SwissProt: Q9DCC8 Mouse
- SwissProt: Q62760 Rat
- Unigene: 533192 Human
see all -
Alternative names
- KIAA0016 antibody
- MAS20 antibody
- MGC117367 antibody
see all
Images
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All lanes : Anti-TOMM20 antibody [EPR15581-39] - Mouse IgG1 (Chimeric) (ab283317) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 3 : C6 (rat glial tumor glial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDaThis data was developed using ab283317 the same antibody clone in a different buffer formulation.
Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: Lane 1: 70 seconds; Lane 2-3: 59 seconds
Lane 2-3 of this blot was developed using a higher sensitivity ECL substrate.
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This data was developed using ab283317 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human liver. The section was incubated with ab283317 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. Counterstained with Hematoxylin.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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This data was developed using ab283317 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse kidney. The section was incubated with ab283317 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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This data was developed using ab283317 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat kidney. The section was incubated with ab283317 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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This data was developed using ab283317 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilised mouse small intestine labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Positive staining on mouse small intestine. Nuclear counterstain: DAPI.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
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This data was developed using ab283317 the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilised rat small intestine labeling TOMM20 with ab283317 at 1/500 dilution (2.16 ug/ml) followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. Positive staining on rat small intestine. Nuclear counterstain: DAPI.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)
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This data was developed using ab283317 the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling TOMM20 with ab283317 at 1/500 dilution (2.16 μg/ml), followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody at 1/200 dilution was used to counterstain tubulin, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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This data was developed using ab283317 the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling TOMM20 with ab283317 at 1/1000 dilution (0.1μg) (Red) compared with a Mouse monoclonal Ig (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab283339 has not yet been referenced specifically in any publications.