The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling TOMM20 (green) with ab186734 at 1/250 dilution. Alexa Fluor®488-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
Western blot - Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734) at 1/5000 dilution + HepG2 cell lysate at 20 µg
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 16 kDa Observed band size: 16 kDa
Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue, labeling TOMM20 with ab186734 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue tissue, labeling TOMM20 with ab186734 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.