Product nameAnti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (HRP)
See all TOMM20 primary antibodies
DescriptionRabbit monoclonal [EPR15581-39] to TOMM20 - Mitochondrial Marker (HRP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human TOMM20 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q15388
- WB: HepG2, HeLa cell lysates. IHC-P: FFPE normal human colon tissue sections.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (ab186734)
- Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (Alexa Fluor® 488) (ab205486)
- Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (Alexa Fluor® 647) (ab205487)
- Anti-TOMM20 antibody [EPR15581-39] (Alexa Fluor® 555) (ab214409)
- Anti-TOMM20 antibody [EPR15581-39] - BSA and Azide free (ab220822)
Our Abpromise guarantee covers the use of ab205796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 16 kDa).|
FunctionCentral component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.
Sequence similaritiesBelongs to the Tom20 family.
Cellular localizationMitochondrion outer membrane.
- Information by UniProt
- KIAA0016 antibody
- MAS20 antibody
- MGC117367 antibody
IHC image of TOMM20 staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab205796, 1/250 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : Anti-TOMM20 antibody [EPR15581-39] - Mitochondrial Marker (HRP) (ab205796) at 1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205796 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab205796 has not yet been referenced specifically in any publications.