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    tomm34-antibody-ab103585.pdf

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Signal Transduction Protein Trafficking Organelle Proteins
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Anti-TOMM34 antibody (ab103585)

  • Datasheet
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Western blot - Anti-TOMM34 antibody (ab103585)
  • Western blot - Anti-TOMM34 antibody (ab103585)
  • Western blot - Anti-TOMM34 antibody (ab103585)

Key features and details

  • Rabbit polyclonal to TOMM34
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human TOMM34 protein (ab156737)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-TOMM34 antibody
    See all TOMM34 primary antibodies
  • Description

    Rabbit polyclonal to TOMM34
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-309 of Human TOMM34 (NP_006800.2).

  • Positive control

    • Mouse testis tissue lysate. Hela NE.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human TOMM34 protein (ab156737)

Applications

Our Abpromise guarantee covers the use of ab103585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.

Target

  • Function

    Plays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the Tom34 family.
    Contains 6 TPR repeats.
  • Cellular localization

    Cytoplasm. Mitochondrion outer membrane.
  • Target information above from: UniProt accession Q15785 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10953 Human
    • Entrez Gene: 67145 Mouse
    • Omim: 616049 Human
    • SwissProt: Q15785 Human
    • SwissProt: Q9CYG7 Mouse
    • Unigene: 517066 Human
    • Unigene: 23173 Mouse
    • Alternative names

      • hTom34 antibody
      • HTOM34P antibody
      • Mitochondrial import receptor subunit TOM34 antibody
      • OTTHUMP00000031079 antibody
      • Outer mitochondrial membrane translocase (34kD) antibody
      • TOM 34 antibody
      • TOM34 antibody
      • TOM34_HUMAN antibody
      • TOMM 34 antibody
      • TOMM34 antibody
      • Translocase of outer membrane 34 kDa subunit antibody
      • Translocase of outer mitochondrial membrane 34 antibody
      • URCC 3 antibody
      • URCC3 antibody
      see all

    Images

    • Western blot - Anti-TOMM34 antibody (ab103585)
      Western blot - Anti-TOMM34 antibody (ab103585)
      Anti-TOMM34 antibody (ab103585) at 1/500 dilution + Mouse testis tissue lysate at 50 µg

      Predicted band size: 35 kDa

    • Western blot - Anti-TOMM34 antibody (ab103585)
      Western blot - Anti-TOMM34 antibody (ab103585)
      All lanes : Anti-TOMM34 antibody (ab103585) at 1/500 dilution

      Lane 1 : TOMM34 transfected 293T cell lysate
      Lane 2 : 293T non-transfected cell lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 35 kDa

    • Western blot - Anti-TOMM34 antibody (ab103585)
      Western blot - Anti-TOMM34 antibody (ab103585)
      Anti-TOMM34 antibody (ab103585) at 1/500 dilution + HeLa NE at 50 µg

      Predicted band size: 35 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab103585? Please let us know so that we can cite the reference in this datasheet.

    ab103585 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-TOMM34 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Heart)
    Permeabilization
    Yes - Tween-20
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 29 2016

    Western blot abreview for Anti-TOMM34 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 29 2016

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