Overview

  • Product name
    Anti-TOMM34 antibody
    See all TOMM34 primary antibodies
  • Description
    Rabbit polyclonal to TOMM34
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-309 of Human TOMM34 (NP_006800.2).

  • Positive control
    • Mouse testis tissue lysate. Hela NE.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.

Target

  • Function
    Plays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the Tom34 family.
    Contains 6 TPR repeats.
  • Cellular localization
    Cytoplasm. Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • hTom34 antibody
      • HTOM34P antibody
      • Mitochondrial import receptor subunit TOM34 antibody
      • OTTHUMP00000031079 antibody
      • Outer mitochondrial membrane translocase (34kD) antibody
      • TOM 34 antibody
      • TOM34 antibody
      • TOM34_HUMAN antibody
      • TOMM 34 antibody
      • TOMM34 antibody
      • Translocase of outer membrane 34 kDa subunit antibody
      • Translocase of outer mitochondrial membrane 34 antibody
      • URCC 3 antibody
      • URCC3 antibody
      see all

    Images

    • Western blot - Anti-TOMM34 antibody (ab103585)
      Western blot - Anti-TOMM34 antibody (ab103585)
      Anti-TOMM34 antibody (ab103585) at 1/500 dilution + Mouse testis tissue lysate at 50 µg

      Predicted band size: 35 kDa

    • Western blot - Anti-TOMM34 antibody (ab103585)
      Western blot - Anti-TOMM34 antibody (ab103585)
      All lanes : Anti-TOMM34 antibody (ab103585) at 1/500 dilution

      Lane 1 : TOMM34 transfected 293T cell lysate
      Lane 2 : 293T non-transfected cell lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 35 kDa

    • Western blot - Anti-TOMM34 antibody (ab103585)
      Western blot - Anti-TOMM34 antibody (ab103585)
      Anti-TOMM34 antibody (ab103585) at 1/500 dilution + HeLa NE at 50 µg

      Predicted band size: 35 kDa

    References

    ab103585 has not yet been referenced specifically in any publications.

    Publishing research using ab103585? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Submit a review Submit a question

    2 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Frozen sections) abreview for Anti-TOMM34 antibody

     Good
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Heart)
    Permeabilization
    Yes - Tween-20
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Aug 29 2016

    Western blot abreview for Anti-TOMM34 antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    Heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Jul 29 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up