Recombinant
RabMAb

Recombinant Anti-TOMM40 antibody [EPR6932(2)] (Alexa Fluor® 488) (ab211401)

Overview

  • Product name

    Anti-TOMM40 antibody [EPR6932(2)] (Alexa Fluor® 488)
    See all TOMM40 primary antibodies
  • Description

    Rabbit monoclonal [EPR6932(2)] to TOMM40 (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human TOMM40 aa 100-200. The exact sequence is proprietary.
    Database link: O96008

  • Positive control

    • ICC/IF: HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab211401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Target

  • Function

    Channel-forming protein essential for import of protein precursors into mitochondria.
  • Sequence similarities

    Belongs to the Tom40 family.
  • Cellular localization

    Mitochondrion outer membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C19orf1 antibody
    • D19S1177E antibody
    • Haymaker protein antibody
    • Mitochondrial import receptor subunit TOM40 homolog antibody
    • Mitochondrial outer membrane protein antibody
    • Mitochondrial outer membrane protein TOM40 antibody
    • p38.5 antibody
    • PER EC1 antibody
    • PEREC1 antibody
    • Probable mitochondrial import receptor subunit TOM40 homolog antibody
    • Protein Haymaker antibody
    • TOM40 antibody
    • TOM40_HUMAN antibody
    • TOMM40 antibody
    • Translocase of outer membrane 40 kDa subunit homolog antibody
    • Translocase of outer mitochondrial membrane 40 antibody
    • Translocase of outer mitochondrial membrane 40 homolog (yeast) antibody
    • Translocase of outer mitochondrial membrane 40 homolog antibody
    • Translocase of outer mitochondrial membrane 40, yeast, homolog of antibody
    see all

Images

  • ab211401 staining TOMM40 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab211401 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (pseudocolored in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

References

ab211401 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab211401.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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