Product nameAnti-Tomosyn antibody
See all Tomosyn primary antibodies
DescriptionRabbit polyclonal to Tomosyn
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- This antibody gave a positive signal in the following tissues lysates: Rat Brain, Mouse Brain and Human Brain
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab45228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).|
RelevanceTomosyn plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release by inhibiting the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane, via the displacement of munc18 from syntaxin-1. There are three splice variants of tomosyn designated m-tomosyn, b-tomosyn and s-tomosyn. Although b-tomosyn is ubiquitously expressed, s-tomosyn and m-tomosyn are expressed primarily in brain.
Cellular localizationCell Membrane and Cytoplasmic. Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles
- FLJ30922 antibody
- Lethal(2) giant larvae protein homolog 3 antibody
- LGL3 antibody
All lanes : Anti-Tomosyn antibody (ab45228) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 128 kDa
Additional bands at: 30 kDa, 52 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
ab45228 has not yet been referenced specifically in any publications.