Key features and details
- Rabbit polyclonal to Tomosyn
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Tomosyn antibody
See all Tomosyn primary antibodies
DescriptionRabbit polyclonal to Tomosyn
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Tomosyn aa 850-950 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissues lysates: Rat Brain, Mouse Brain and Human Brain
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab45228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).|
RelevanceTomosyn plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release by inhibiting the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane, via the displacement of munc18 from syntaxin-1. There are three splice variants of tomosyn designated m-tomosyn, b-tomosyn and s-tomosyn. Although b-tomosyn is ubiquitously expressed, s-tomosyn and m-tomosyn are expressed primarily in brain.
Cellular localizationCell Membrane and Cytoplasmic. Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles
- FLJ30922 antibody
- Lethal(2) giant larvae protein homolog 3 antibody
- LGL3 antibody
All lanes : Anti-Tomosyn antibody (ab45228) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 128 kDa
Additional bands at: 30 kDa, 52 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
ab45228 has not yet been referenced specifically in any publications.