Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)
![Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)](https://www.abcam.com/ps/products/183/ab183520/Images/ab183520-215002-anti-top3b-antibody-ep7779-c-terminal-western-blot.jpg "Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)")
Key features and details
- Rabbit monoclonal [EP7779] to TOP3B - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-TOP3B antibody [EP7779] - C-terminal
See all TOP3B primary antibodies -
Description
Rabbit monoclonal [EP7779] to TOP3B - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab199552) -
Positive control
- K562, C6, Raw264.7, PC-12, NIH 3T3 and HepG2 cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP7779 -
Isotype
IgG -
Research areas
Associated products
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human TOP3B peptide (ab199552).
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| Notes |
|---|
|
WB
1/1000 - 1/2000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human TOP3B peptide (ab199552). |
Target
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Function
Possesses negatively supercoiled DNA relaxing activity. -
Tissue specificity
Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas. -
Sequence similarities
Belongs to the prokaryotic type I/III topoisomerase family.
Contains 1 Toprim domain. - Information by UniProt
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Database links
- Entrez Gene: 8940 Human
- Entrez Gene: 21976 Mouse
- Entrez Gene: 287930 Rat
- Omim: 603582 Human
- SwissProt: O95985 Human
- SwissProt: Q9Z321 Mouse
- Unigene: 436401 Human
- Unigene: 326089 Mouse
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Alternative names
- DNA topoisomerase 3 beta 1 antibody
- DNA topoisomerase 3-beta-1 antibody
- DNA topoisomerase III beta 1 antibody
see all
Images
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Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)All lanes : Anti-TOP3B antibody [EP7779] - C-terminal (ab183520) at 1/2000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 97 kDa -
![Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)](https://www.abcam.com/ps/products/183/ab183520/Images/ab183520-215392-anti-top3b-antibody-ep7779-c-terminal-western-blot.jpg)
Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)All lanes :
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 97 kDa
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (4)
ab183520 has been referenced in 4 publications.
- Chen J et al. CT2-3, a novel magnolol analogue suppresses NSCLC cells through triggering cell cycle arrest and apoptosis. Bioorg Med Chem 28:115352 (2020). PubMed: 32044229
- Saha S et al. DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA- and DNA-Protein Crosslinks. Cell Rep 33:108569 (2020). PubMed: 33378676
- Prasanth KR et al. Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses. bioRxiv N/A:N/A (2020). PubMed: 32511318
- Huang L et al. Arginine methylation of the C-terminus RGG motif promotes TOP3B topoisomerase activity and stress granule localization. Nucleic Acids Res 46:3061-3074 (2018). PubMed: 29471495
