Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)
![Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)](https://www.abcam.com/ps/products/183/ab183520/Images/ab183520-215002-anti-top3b-antibody-ep7779-c-terminal-western-blot.jpg "Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)")
Key features and details
- Rabbit monoclonal [EP7779] to TOP3B - C-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-TOP3B antibody [EP7779] - C-terminal
See all TOP3B primary antibodies -
Description
Rabbit monoclonal [EP7779] to TOP3B - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
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Positive control
- K562, C6, Raw264.7, PC-12, NIH 3T3 and HepG2 cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP7779 -
Isotype
IgG -
Research areas
Associated products
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab183520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human TOP3B peptide (ab199552). |
Target
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Function
Possesses negatively supercoiled DNA relaxing activity. -
Tissue specificity
Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas. -
Sequence similarities
Belongs to the prokaryotic type I/III topoisomerase family.
Contains 1 Toprim domain. - Information by UniProt
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Database links
- Entrez Gene: 8940 Human
- Entrez Gene: 21976 Mouse
- Entrez Gene: 287930 Rat
- Omim: 603582 Human
- SwissProt: O95985 Human
- SwissProt: Q9Z321 Mouse
- Unigene: 436401 Human
- Unigene: 326089 Mouse
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Alternative names
- DNA topoisomerase 3 beta 1 antibody
- DNA topoisomerase 3-beta-1 antibody
- DNA topoisomerase III beta 1 antibody
see all
Images
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Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)All lanes : Anti-TOP3B antibody [EP7779] - C-terminal (ab183520) at 1/2000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 97 kDa -
![Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)](https://www.abcam.com/ps/products/183/ab183520/Images/ab183520-215392-anti-top3b-antibody-ep7779-c-terminal-western-blot.jpg)
Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)All lanes :
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 97 kDa
Datasheets and documents
Certificate of Compliance
References (1)
ab183520 has been referenced in 1 publication.
- Huang L et al. Arginine methylation of the C-terminus RGG motif promotes TOP3B topoisomerase activity and stress granule localization. Nucleic Acids Res 46:3061-3074 (2018). PubMed: 29471495
