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  1. Link

    top3b-antibody-ep7779-c-terminal-ab183520.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis Topoisomerases
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RabMAb

Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)

  • Datasheet
  • Certificate of Compliance
Reviews (1) Submit a question References (4)

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Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)
  • Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)

Key features and details

  • Rabbit monoclonal [EP7779] to TOP3B - C-terminal
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-TOP3B antibody [EP7779] - C-terminal
    See all TOP3B primary antibodies
  • Description

    Rabbit monoclonal [EP7779] to TOP3B - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab199552)

  • Positive control

    • K562, C6, Raw264.7, PC-12, NIH 3T3 and HepG2 cell lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP7779
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Topoisomerases

Associated products

  • Immunizing Peptide (Blocking)

    • Human TOP3B peptide (ab199552)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)
    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)
    • K-562 whole cell lysate (ab7911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/2000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human TOP3B peptide (ab199552).
Notes
WB
1/1000 - 1/2000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human TOP3B peptide (ab199552).

Target

  • Function

    Possesses negatively supercoiled DNA relaxing activity.
  • Tissue specificity

    Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.
  • Sequence similarities

    Belongs to the prokaryotic type I/III topoisomerase family.
    Contains 1 Toprim domain.
  • Target information above from: UniProt accession O95985 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8940 Human
    • Entrez Gene: 21976 Mouse
    • Entrez Gene: 287930 Rat
    • Omim: 603582 Human
    • SwissProt: O95985 Human
    • SwissProt: Q9Z321 Mouse
    • Unigene: 436401 Human
    • Unigene: 326089 Mouse
    • Alternative names

      • DNA topoisomerase 3 beta 1 antibody
      • DNA topoisomerase 3-beta-1 antibody
      • DNA topoisomerase III beta 1 antibody
      • DNA topoisomerase III beta-1 antibody
      • FLJ39376 antibody
      • TOP 3B antibody
      • TOP3B antibody
      • TOP3B_HUMAN antibody
      • TOP3B1 antibody
      • Topo III beta antibody
      • Topoisomerase (DNA) III beta antibody
      • Topoisomerase DNA III beta antibody
      • Topoisomerase III beta antibody
      see all

    Images

    • Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)
      Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)
      All lanes : Anti-TOP3B antibody [EP7779] - C-terminal (ab183520) at 1/2000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 97 kDa

    • Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)
      Western blot - Anti-TOP3B antibody [EP7779] - C-terminal (ab183520)
      All lanes :

      Lane 1 : C6 cell lysate
      Lane 2 : Raw264.7 cell lysate
      Lane 3 : PC-12 cell lysate
      Lane 4 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 97 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (4)

    Publishing research using ab183520? Please let us know so that we can cite the reference in this datasheet.

    ab183520 has been referenced in 4 publications.

    • Chen J  et al. CT2-3, a novel magnolol analogue suppresses NSCLC cells through triggering cell cycle arrest and apoptosis. Bioorg Med Chem 28:115352 (2020). PubMed: 32044229
    • Saha S  et al. DNA and RNA Cleavage Complexes and Repair Pathway for TOP3B RNA- and DNA-Protein Crosslinks. Cell Rep 33:108569 (2020). PubMed: 33378676
    • Prasanth KR  et al. Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses. bioRxiv N/A:N/A (2020). PubMed: 32511318
    • Huang L  et al. Arginine methylation of the C-terminus RGG motif promotes TOP3B topoisomerase activity and stress granule localization. Nucleic Acids Res 46:3061-3074 (2018). PubMed: 29471495

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-TOP3B antibody [EP7779] - C-terminal

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Neuro2A (N2A) cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Neuro2A (N2A) cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    DR. Michael Kearse

    Verified customer

    Submitted Dec 24 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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