Key features and details
- Rabbit polyclonal to TopBP1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
Product nameAnti-TopBP1 antibody
See all TopBP1 primary antibodies
DescriptionRabbit polyclonal to TopBP1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human, African green monkey
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide (Human) - which represents a portion of human Topoisomerase (DNA) II Binding Protein I encoded within exon 28.
- HeLa cells and HCT116
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ChIP Related Products
Our Abpromise guarantee covers the use of ab2402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 173.3 kDa).|
|IHC-P||Use a concentration of 4 µg/ml.|
FunctionRequired for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR.
Tissue specificityHighly expressed in heart, brain, placenta, lung and kidney.
Sequence similaritiesContains 8 BRCT domains.
modificationsPhosphorylated on serine and threonine residues in response to X-ray irradiation.
Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination.
Cellular localizationNucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome. Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner.
- Information by UniProt
- DNA topoisomerase 2 binding protein 1 antibody
- DNA topoisomerase 2-binding protein 1 antibody
- DNA topoisomerase II binding protein 1 antibody
ab2402 has been referenced in 30 publications.
- Deshar R et al. RNF8 mediates NONO degradation following UV-induced DNA damage to properly terminate ATR-CHK1 checkpoint signaling. Nucleic Acids Res 47:762-778 (2019). PubMed: 30445466
- Wu M et al. A PLK1 kinase inhibitor enhances the chemosensitivity of cisplatin by inducing pyroptosis in oesophageal squamous cell carcinoma. EBioMedicine 41:244-255 (2019). PubMed: 30876762
- Michelena J et al. Basal CHK1 activity safeguards its stability to maintain intrinsic S-phase checkpoint functions. J Cell Biol 218:2865-2875 (2019). PubMed: 31366665
- Leimbacher PA et al. MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis. Mol Cell 74:571-583.e8 (2019). PubMed: 30898438
- Fragkos M et al. Dicer prevents genome instability in response to replication stress. Oncotarget 10:4407-4423 (2019). PubMed: 31320994