Anti-TopBP1 antibody (ab2402)
Key features and details
- Rabbit polyclonal to TopBP1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TopBP1 antibody
See all TopBP1 primary antibodies -
Description
Rabbit polyclonal to TopBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human TopBP1 aa 1400-1500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_008958.1 (GeneID 11073).
Database link: Q92547 -
Positive control
- WB: HeLa, HEK-293T, TCMK-1, NIH/3T3 and Jurkat whole cell lysate. IP: TopBP1 in HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (8) |
1/2000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 173.3 kDa).
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IP | (2) |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 173.3 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. -
Tissue specificity
Highly expressed in heart, brain, placenta, lung and kidney. -
Sequence similarities
Contains 8 BRCT domains. -
Post-translational
modificationsPhosphorylated on serine and threonine residues in response to X-ray irradiation.
Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination. -
Cellular localization
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome. Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner. - Information by UniProt
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Database links
- Entrez Gene: 11073 Human
- Entrez Gene: 235559 Mouse
- Omim: 607760 Human
- SwissProt: Q92547 Human
- SwissProt: Q6ZQF0 Mouse
- Unigene: 593379 Human
- Unigene: 259893 Mouse
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Alternative names
- DNA topoisomerase 2 binding protein 1 antibody
- DNA topoisomerase 2-binding protein 1 antibody
- DNA topoisomerase II binding protein 1 antibody
see all
Images
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All lanes : Anti-Cytokeratin 10 antibody [LHP1] (ab2409) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 173.3 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Antibodies: ab2409 used for IP at 3 µg per reaction.
Two lots tested (Lanes 1 and 2).
Lane 3 is Control IgG.
For blotting immunoprecipitated TopBP1, ab2409 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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All lanes : Anti-Cytokeratin 10 antibody [LHP1] (ab2409) at 0.1 µg/ml
Lane 1 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 173.3 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab2402 has been referenced in 40 publications.
- Balbo Pogliano C et al. The CDK1-TOPBP1-PLK1 axis regulates the Bloom's syndrome helicase BLM to suppress crossover recombination in somatic cells. Sci Adv 8:eabk0221 (2022). PubMed: 35119917
- De Marco Zompit M et al. The CIP2A-TOPBP1 complex safeguards chromosomal stability during mitosis. Nat Commun 13:4143 (2022). PubMed: 35842428
- Bradbury A et al. The Role of ATR Inhibitors in Ovarian Cancer: Investigating Predictive Biomarkers of Response. Cells 11:N/A (2022). PubMed: 35954206
- Adam S et al. The CIP2A-TOPBP1 axis safeguards chromosome stability and is a synthetic lethal target for BRCA-mutated cancer. Nat Cancer 2:1357-1371 (2021). PubMed: 35121901
- Ishimoto R et al. SLX4-XPF mediates DNA damage responses to replication stress induced by DNA-protein interactions. J Cell Biol 220:N/A (2021). PubMed: 33347546