Overview

  • Product name

    Anti-Topoisomerase I antibody [23B11]
    See all Topoisomerase I primary antibodies
  • Description

    Mouse monoclonal [23B11] to Topoisomerase I
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Xenopus laevis, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human Topoisomerase I aa 699-725 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    MKLEVQATDREENKQIALGTSKLNYLD


    Database link: P11387

  • Positive control

    • Whole cell lysates of serum starved HeLa, HepG2, HEK293, SH-SY5Y, MDCK PC12, CMT 93, Neuro 2A and 3T3 tumor cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab200869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 91 kDa.

Target

  • Function

    The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
  • Involvement in disease

    Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
  • Sequence similarities

    Belongs to the eukaryotic type I topoisomerase family.
  • Post-translational
    modifications

    Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
  • Cellular localization

    Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA topoisomerase 1 antibody
    • DNA topoisomerase I antibody
    • NUP98 fusion gene antibody
    • TOP 1 antibody
    • TOP I antibody
    • TOP1 antibody
    • TOP1_HUMAN antibody
    • TOPI antibody
    • Topoisomerase (DNA) I antibody
    • Topoisomerase 1 antibody
    • Topoisomerase1 antibody
    • TopoisomeraseI antibody
    • Type I DNA topoisomerase antibody
    see all

Images

  • All lanes : Anti-Topoisomerase I antibody [23B11] (ab200869) at 0.5 µg/ml

    Lane 1 : Whole cell lysate of serum starved HeLa cells
    Lane 2 : Whole cell lysate of serum starved HepG2 cells
    Lane 3 : Whole cell lysate of serum starved HEK293 cells
    Lane 4 : Whole cell lysate of serum starved SH-SY5Y cells
    Lane 5 : Whole cell lysate of serum starved MDCK cells
    Lane 6 : Whole cell lysate of serum starved PC12 cells
    Lane 7 : Whole cell lysate of serum starved CMT 93 cells
    Lane 8 : Whole cell lysate of serum starved Neuro 2A cells
    Lane 9 : Whole cell lysate of serum starved 3T3 tumor cells

    Lysates/proteins at 20000 cells per lane.

    Developed using the ECL technique.

    Predicted band size: 91 kDa


    Exposure time: 30 seconds


    PVDF membrane.

References

ab200869 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (fibroblast)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
30 µg
Specification
fibroblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Apr 28 2017

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