Key features and details
- Mouse monoclonal [23B11] to Topoisomerase I
- Suitable for: WB
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG1
Product nameAnti-Topoisomerase I antibody [23B11]
See all Topoisomerase I primary antibodies
DescriptionMouse monoclonal [23B11] to Topoisomerase I
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Dog, Human
Predicted to work with: Xenopus laevis, Chinese hamster
- Whole cell lysates of serum starved HeLa, HepG2, HEK293, SH-SY5Y, MDCK PC12, CMT 93, Neuro 2A and 3T3 tumor cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.09% Sodium azide
With PEG and sucrose.
Concentration information loading...
Our Abpromise guarantee covers the use of ab200869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Predicted molecular weight: 91 kDa.|
FunctionThe reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Involvement in diseaseNote=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
Sequence similaritiesBelongs to the eukaryotic type I topoisomerase family.
modificationsSumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
- Information by UniProt
- DNA topoisomerase 1 antibody
- DNA topoisomerase I antibody
- NUP98 fusion gene antibody
All lanes : Anti-Topoisomerase I antibody [23B11] (ab200869) at 0.5 µg/ml
Lane 1 : Whole cell lysate of serum starved HeLa cells
Lane 2 : Whole cell lysate of serum starved HepG2 cells
Lane 3 : Whole cell lysate of serum starved HEK293 cells
Lane 4 : Whole cell lysate of serum starved SH-SY5Y cells
Lane 5 : Whole cell lysate of serum starved MDCK cells
Lane 6 : Whole cell lysate of serum starved PC12 cells
Lane 7 : Whole cell lysate of serum starved CMT 93 cells
Lane 8 : Whole cell lysate of serum starved Neuro 2A cells
Lane 9 : Whole cell lysate of serum starved 3T3 tumor cells
Lysates/proteins at 20000 cells per lane.
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 30 seconds
ab200869 has not yet been referenced specifically in any publications.