Key features and details
- Rabbit polyclonal to Topoisomerase I
- Suitable for: WB, ICC/IF, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Topoisomerase I antibody
See all Topoisomerase I primary antibodies
DescriptionRabbit polyclonal to Topoisomerase I
SpecificityAb28432 recognises Topoisomerase I.
Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
Species reactivityReacts with: Human
Recombinant full length Topoisomerase I protein (Human).
- HeLa nuclear extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.9
Concentration information loading...
PurityProtein A purified
Purification notesAb28432 is affinity purified.
Our Abpromise guarantee covers the use of ab28432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Predicted molecular weight: 91 kDa.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionThe reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Involvement in diseaseNote=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
Sequence similaritiesBelongs to the eukaryotic type I topoisomerase family.
modificationsSumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
- Information by UniProt
- DNA topoisomerase 1 antibody
- DNA topoisomerase I antibody
- NUP98 fusion gene antibody
ICC/IF image of ab28432 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ICC/IF image of ab28432 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab28432 has been referenced in 9 publications.
- Jensen NF et al. Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a new pair of resistance-associated mutations. J Exp Clin Cancer Res 35:56 (2016). PubMed: 27029323
- Xia Q et al. Characterization of the transcriptional machinery bound across the widely presumed type 2 diabetes causal variant, rs7903146, within TCF7L2. Eur J Hum Genet N/A:N/A (2014). Human . PubMed: 24667787
- Kiianitsa K & Maizels N Ultrasensitive isolation, identification and quantification of DNA-protein adducts by ELISA-based RADAR assay. Nucleic Acids Res 42:e108 (2014). PubMed: 24914050
- Kiianitsa K & Maizels N A rapid and sensitive assay for DNA-protein covalent complexes in living cells. Nucleic Acids Res 41:e104 (2013). WB ; Human . PubMed: 23519618
- Montariello D et al. p63 involvement in poly(ADP-ribose) polymerase 1 signaling of topoisomerase I-dependent DNA damage in carcinoma cells. Biochem Pharmacol 85:999-1006 (2013). Human . PubMed: 23376119
- Pfister TD et al. Development and validation of an immunoassay for quantification of topoisomerase I in solid tumor tissues. PLoS One 7:e50494 (2012). Human . PubMed: 23284638
- D'Onofrio G et al. Poly(ADP-ribose) polymerase signaling of topoisomerase 1-dependent DNA damage in carcinoma cells. Biochem Pharmacol 81:194-202 (2011). WB ; Human . PubMed: 20875401
- Pfister TD et al. Topoisomerase I levels in the NCI-60 cancer cell line panel determined by validated ELISA and microarray analysis and correlation with indenoisoquinoline sensitivity. Mol Cancer Ther 8:1878-84 (2009). ELISA ; Human . PubMed: 19584232
- Cohen AA et al. Dynamic proteomics of individual cancer cells in response to a drug. Science 322:1511-6 (2008). WB ; Human . PubMed: 19023046