Overview

  • Product name

    Anti-Topoisomerase I antibody - C-terminal
    See all Topoisomerase I primary antibodies
  • Description

    Rabbit polyclonal to Topoisomerase I - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human Topoisomerase I (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: P11387

  • Positive control

    • WB: HEK-293T whole cell and nuclear extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab227596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 91 kDa.

Target

  • Function

    The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
  • Involvement in disease

    Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
  • Sequence similarities

    Belongs to the eukaryotic type I topoisomerase family.
  • Post-translational
    modifications

    Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
  • Cellular localization

    Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
  • Information by UniProt
  • Database links

  • Alternative names

    • DNA topoisomerase 1 antibody
    • DNA topoisomerase I antibody
    • NUP98 fusion gene antibody
    • TOP 1 antibody
    • TOP I antibody
    • TOP1 antibody
    • TOP1_HUMAN antibody
    • TOPI antibody
    • Topoisomerase (DNA) I antibody
    • Topoisomerase 1 antibody
    • Topoisomerase1 antibody
    • TopoisomeraseI antibody
    • Type I DNA topoisomerase antibody
    see all

Images

  • All lanes : Anti-Topoisomerase I antibody - C-terminal (ab227596) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) nuclear extract

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 91 kDa



    7.5% SDS-PAGE gel.

References

ab227596 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227596.
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