Western blot abreview for Anti-Topoisomerase I antibody - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Neuro-2A)
Loading amount
100 µg
Specification
Neuro-2A
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
StartingBlock T20 (TBS) Blocking Buffer as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 21°C

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: StartingBlock T20 (TBS) Blocking Buffer

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-rabbit IgG, HRP linked
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
15 second(s)
Bands
Specific: 100 kDa Non-specific: 35 kDa
Positive control
K-562 nuclear extract

Additional data

Additional Notes
positive control - K-562 nuclear extract (lane 1), mouse samples (lane 2)

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Submitted May 02 2012

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