Western blot abreview for Anti-Topoisomerase I antibody - ChIP Grade

Inconclusive
Abreviews
Application
Western blot
Sample
Arabidopsis thaliana Tissue lysate - nuclear (Seedlings)
Loading amount
50 µg
Specification
Seedlings
Gel Running Conditions
Reduced Denaturing (8% SDS PAGE)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST

Secondary antibody

Name
Non-Abcam antibody was used: IRDye® 800CW anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/20000

Detection

Detection method
Odyssey Scanner
Exposure
1 second(s)
Bands
Specific: no kDa Non-specific: 50, 70 kDa

Additional data

Abcam response
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this application on this tissue type we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application.
For UNTESTED species and/or applications, we have established a testing discount program. We encourage customers to contact us should they wish to try our products in untested species or applications. With this program, the customer can receive a discount for ONE antibody at the full price (100%) for testing and leaving an Abreview. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Mr. Sascha Waidmann

Verified customer

Submitted Sep 05 2012

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