Western blot abreview for Anti-Topoisomerase I antibody - ChIP Grade

Good
Abreviews
Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Chinese Hamster Cell lysate - whole cell (EM9)
Specification
EM9
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Dako polyclonal Goat anti-Rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Bands
Specific: 100 kDa Non-specific: 60 kDa
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Positive control
Human A431 whole cell extract (100,000 cells)

Additional data

Additional Notes
Lane 1: Human A431 whole cell extract Lane 2: Chinese Hamster EM9 whole cell extract 45 KDa - Actin

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Submitted Nov 18 2014

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