Western blot abreview for Anti-Topoisomerase I antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
50 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Swine anti rabbit
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 90 kDa

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Submitted Nov 01 2016

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