Western blot abreview for Anti-Topoisomerase I antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS Cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
20 µg
Specification
U2OS Cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Licor Blocking Buffer
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG Secondary Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 680

Detection

Detection method
Li-Cor
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 100 kDa
Positive control
Whole cell lysate from U2OS cells

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Submitted Jul 14 2017

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