Human Cell lysate - other (top1 cleavage complex purify by CscL gradient)
Gel Running Conditions
Non-reduced Denaturing (Slot Blot)
DMSO or 10uM CPT
top1 cleavage complex purify by CscL gradient
Licor Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
2 hour(s) and 0 minute(s) · Temperature: 25°C
Non-Abcam antibody was used: 800CW anti Rb (licor Odyssey) Host species: Goat Clonality: Polyclonal Conjugation: IRDye® 800CW
cells were treated with DMSO (line 1). Then, cells were lysed with 1% Sarkozyl (+ Protease inhibitors). Proteins covalently bind to DNA was purify by CsCl and load in slotblot.
16 minute(s) and 0 second(s)
Specific: 1 kDa
cells were treated with 10uM CPT (line 2). Then, cells were lysed with 1% Sarkozyl (+ Protease inhibitors). Proteins covalently bind to DNA was purify by CsCl and load in slotblot.
This experiment is not a classical western blot, since the material is load directly into a nitrocellulose membrane without passing through a acrylamide gel. Therefore, we don't separate the proteins by size.
Ms. Cristina Bernal
Submitted Mar 26 2019
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