Overview

  • Product name

    Anti-Topoisomerase I antibody [EPR5376(2)]
    See all Topoisomerase I primary antibodies
  • Description

    Rabbit monoclonal [EPR5376(2)] to Topoisomerase I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Topoisomerase I aa 700-800. The exact sequence is proprietary.

  • Positive control

    • MCF7, Jurkat, SW480, K562, Ramos, RAW264.7 and PC12 cell lysates; MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 91 kDa).
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
    • Involvement in disease

      Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
    • Sequence similarities

      Belongs to the eukaryotic type I topoisomerase family.
    • Post-translational
      modifications

      Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
    • Cellular localization

      Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
    • Information by UniProt
    • Database links

    • Alternative names

      • DNA topoisomerase 1 antibody
      • DNA topoisomerase I antibody
      • NUP98 fusion gene antibody
      • TOP 1 antibody
      • TOP I antibody
      • TOP1 antibody
      • TOP1_HUMAN antibody
      • TOPI antibody
      • Topoisomerase (DNA) I antibody
      • Topoisomerase 1 antibody
      • Topoisomerase1 antibody
      • TopoisomeraseI antibody
      • Type I DNA topoisomerase antibody
      see all

    Images

    • All lanes : Anti-Topoisomerase I antibody [EPR5376(2)] (ab131166) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : K562 cell lysate
      Lane 5 : Ramos cell lysate
      Lane 6 : RAW264.7 cell lysate
      Lane 7 : PC12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 91 kDa

    • Immunocytochemistry/Immunofluorescence analysis of HaCaT (Human keratinocyte cell line) labelling Topoisomerase I with purified ab131166 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • Immunofluorescent analysis of MCF7 cells labelling Topoisomerase I with ab131166 at 1/250 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab131166 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131166.
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