Product nameAnti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377] (Alexa Fluor® 555)
DescriptionRabbit monoclonal [EPR5377] to Topoisomerase II alpha + Topoisomerase II beta (Alexa Fluor® 555)
ConjugationAlexa Fluor® 555. Ex: 555nm, Em: 565nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Topoisomerase II beta aa 50-150. The exact sequence is proprietary.
Database link: Q02880
- ICC/IF: HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377] (ab109524)
- Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377] (Alexa Fluor® 647) (ab200993)
- Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377] (Alexa Fluor® 488) (ab201504)
- Anti-Topoisomerase II alpha + Topoisomerase II beta antibody [EPR5377] - BSA and Azide free (ab239984)
Our Abpromise guarantee covers the use of ab206605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
Cellular localizationTopoisomerase II alpha: Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm. Topoisomerase II beta: Cytoplasm. Nucleus > nucleolus.
ab206605 staining Topoisomerase II alpha + Topoisomerase II beta in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab206605 at a 1/500 dilution (pseudocolored in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).
ab206605 has not yet been referenced specifically in any publications.