• Product name
    Anti-Topoisomerase II alpha antibody
    See all Topoisomerase II alpha primary antibodies
  • Description
    Rabbit polyclonal to Topoisomerase II alpha
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide representing a portion of human DNA topoisomerase II, alpha isozyme encoded within exon 35 (LocusLink ID 7153).

  • Positive control
    • HeLa cells. IHC-P: FFPE human testis tissue sections.
  • General notes
    Centrifuge tube to remove product from lid.



Our Abpromise guarantee covers the use of ab12318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/30000. Detects a band of approximately 178 kDa (predicted molecular weight: 184 kDa).
IP Use at 2-10 µg/mg of lysate.


  • Function
    Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
  • Sequence similarities
    Belongs to the type II topoisomerase family.
  • Post-translational
    Phosphorylation has no effect on catalytic activity.
  • Cellular localization
    Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha isozyme antibody
    • ATP hydrolyzing DNA topoisomerase II alfa antibody
    • DNA gyrase antibody
    • DNA topoisomerase (ATP hydrolyzing) antibody
    • DNA topoisomerase 2 alpha antibody
    • DNA topoisomerase 2-alpha antibody
    • DNA topoisomerase II 170 kD antibody
    • DNA topoisomerase II alpha isozyme antibody
    • DNA topoisomerase II antibody
    • DNA Topoisomerase2 antibody
    • TOP 2A antibody
    • TOP2 antibody
    • TOP2A antibody
    • TOP2A_HUMAN antibody
    • Topoisomerase DNA II alpha 170kDa antibody
    • TP2A antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryangeal squamous cell carcinoma labelling Topoisomerase II alpha with ab12318 at 1/1000 (1µg/ml). Detection: DAB.
  • IHC image of Topoisomerase II alpha staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12318, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Detection of Human Topo II Alpha by Western Blot and Immunoprecipitation.

    Samples: Whole cell lysate (25 mcg/lane for A; 0.5 mg for IP in B; 25 mcg for Input in B) from HeLa cells. Antibodies: Affinity purified rabbit anti-Topo II alpha antibody BL983 used at the indicated concentrations for WB (A) and IP (B). Control antibody (Ctrl. in A) and antibody for WB of immunoprecipitated Topo II alpha was a monoclonal antibody to Topo II alpha from another source. Detection: Chemiluminescence with exposure times of 5 minutes (A) or 30 seconds (B).


This product has been referenced in:
  • Bansal K  et al. The transcriptional regulator Aire binds to and activates super-enhancers. Nat Immunol 18:263-273 (2017). Read more (PubMed: 28135252) »
  • Zhao M  et al. Differential expression of multidrug resistance-related proteins in adriamycin-resistant (pumc-91/ADM) and parental (pumc-91) human bladder cancer cell lines. Mol Med Rep 14:4741-4746 (2016). Read more (PubMed: 27748815) »
See all 9 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our AbTrial program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information. Or you can find more information about this program at the following link:


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Read More
Western blot
Mouse Cell lysate - whole cell (embryonic fibroblasts)
Loading amount
90 µg
embryonic fibroblasts
250 uM etoposide for 4h, 1 uM camptothecin for 1h
Gel Running Conditions
Reduced Denaturing (4-18% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Jul 07 2011


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