Anti-Topoisomerase II alpha antibody - C-terminal (ab137657)

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Western blot - Anti-Topoisomerase II alpha antibody - C-terminal (ab137657)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody - C-terminal (ab137657)

Key features and details

  • Rabbit polyclonal to Topoisomerase II alpha - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Topoisomerase II alpha antibody - C-terminal
    See all Topoisomerase II alpha primary antibodies

  • Description

    Rabbit polyclonal to Topoisomerase II alpha - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 1415-1509 of Human Topoisomerase II alpha.

  • Positive control

    • 293T, A431 cell lines and H1299 whole cell lysate. SAS Xenograft

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

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Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 174 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 174 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.

Target

  • Function

    Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.

  • Sequence similarities

    Belongs to the type II topoisomerase family.

  • Post-translational
    modifications

    Phosphorylation has no effect on catalytic activity.

  • Cellular localization

    Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • alpha isozyme antibody
      • ATP hydrolyzing DNA topoisomerase II alfa antibody
      • DNA gyrase antibody
      • DNA topoisomerase (ATP hydrolyzing) antibody
      • DNA topoisomerase 2 alpha antibody
      • DNA topoisomerase 2-alpha antibody
      • DNA topoisomerase II 170 kD antibody
      • DNA topoisomerase II alpha isozyme antibody
      • DNA topoisomerase II antibody
      • DNA Topoisomerase2 antibody
      • TOP 2A antibody
      • TOP2 antibody
      • TOP2A antibody
      • TOP2A_HUMAN antibody
      • Topoisomerase DNA II alpha 170kDa antibody
      • TP2A antibody
      see all

    Images

    • Western blot - Anti-Topoisomerase II alpha antibody - C-terminal (ab137657)
      Western blot - Anti-Topoisomerase II alpha antibody - C-terminal (ab137657)
      Anti-Topoisomerase II alpha antibody - C-terminal (ab137657) at 1/3000 dilution + H1299 whole cell lysate
      at 30 µg

      Predicted band size: 174 kDa



      5% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody - C-terminal (ab137657)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody - C-terminal (ab137657)
      Immunohistochemical analysis of paraffin-embedded SAS Xenograft labelling Topoisomerase II alpha with ab137657 at 1/500 dilution.

    References (0)

    Publishing research using ab137657? Please let us know so that we can cite the reference in this datasheet.

    ab137657 has not yet been referenced specifically in any publications.

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