Anti-Topoisomerase II alpha antibody - C-terminal (ab137657)
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Overview
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Product name
Anti-Topoisomerase II alpha antibody - C-terminal
See all Topoisomerase II alpha primary antibodies -
Description
Rabbit polyclonal to Topoisomerase II alpha - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within C terminal amino acids 1415-1509 of Human Topoisomerase II alpha.
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Positive control
- 293T, A431 cell lines and H1299 whole cell lysate. SAS Xenograft
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 174 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible. |
Target
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Function
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. -
Sequence similarities
Belongs to the type II topoisomerase family. -
Post-translational
modificationsPhosphorylation has no effect on catalytic activity. -
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7153 Human
- Omim: 126430 Human
- SwissProt: P11388 Human
- Unigene: 156346 Human
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Alternative names
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
see all
Images
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Western blot - Anti-Topoisomerase II alpha antibody - C-terminal (ab137657)Anti-Topoisomerase II alpha antibody - C-terminal (ab137657) at 1/3000 dilution + H1299 whole cell lysate
at 30 µg
Predicted band size: 174 kDa
5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody - C-terminal (ab137657)Immunohistochemical analysis of paraffin-embedded SAS Xenograft labelling Topoisomerase II alpha with ab137657 at 1/500 dilution.
References
ab137657 has not yet been referenced specifically in any publications.
