Anti-Topoisomerase II alpha antibody [EP1102Y] Recombinant (ab52934)

Product name

Anti-Topoisomerase II alpha antibody [EP1102Y]
See all Topoisomerase II alpha primary antibodies
Description

Rabbit monoclonal [EP1102Y] to Topoisomerase II alpha
Host species

Rabbit
Specificity

Please be aware that the antibody provided positive results in cell lysate samples but negative results in tissue lysate samples.
Tested applications

Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human Topoisomerase II alpha (C terminal). The exact sequence is proprietary.
Positive control
- HeLa cell lysate; human breast carcinoma tissue. WB: Hela, Jurkat, Neuro-2a and PC-12 whole cell lysate; Human breast cancer, Mouse thymus lysate, Rat thymus lysate and Rat testis lysate
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 5.39 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1102Y
Isotype

IgG
Research areas

Alternative Versions
- Anti-Topoisomerase II alpha antibody [EP1102Y] - BSA and Azide free (ab181828)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Positive Controls
- HeLa nuclear extract lysate (ab14655)

Our Abpromise guarantee covers the use of ab52934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/10000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
IP		1/50.
IHC-P		Use at an assay dependent concentration.

Application notes

Is unsuitable for Flow Cyt or ICC.

Function

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
Sequence similarities

Belongs to the type II topoisomerase family.
Post-translational
modifications

Phosphorylation has no effect on catalytic activity.
Cellular localization

Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
Target information above from: UniProt accession P11388 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7153 Human
- Entrez Gene: 21973 Mouse
- Entrez Gene: 360243 Rat
- Omim: 126430 Human
- SwissProt: P11388 Human
- SwissProt: Q01320 Mouse
- SwissProt: P41516 Rat
- Unigene: 156346 Human
- Unigene: 4237 Mouse
- Unigene: 90996 Rat
see all
Alternative names
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
- DNA topoisomerase (ATP hydrolyzing) antibody
- DNA topoisomerase 2 alpha antibody
- DNA topoisomerase 2-alpha antibody
- DNA topoisomerase II 170 kD antibody
- DNA topoisomerase II alpha isozyme antibody
- DNA topoisomerase II antibody
- DNA Topoisomerase2 antibody
- TOP 2A antibody
- TOP2 antibody
- TOP2A antibody
- TOP2A_HUMAN antibody
- Topoisomerase DNA II alpha 170kDa antibody
- TP2A antibody
see all

Western blot - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

All lanes : Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human acute T cell leukemia) whole cell lysate
Lane 3 : Neuro-2a (mouse neuroblastoma) whole cell lysate
Lane 4 : Human breast cancer lysate
Lane 5 : Mouse thymus lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 7 : Rat thymus lysate
Lane 8 : Rat testis lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 174 kDa
Observed band size: 174 kDa

Exposure time:

Lanes 1-3: 1 second.
Lanes 4-7: 3 minutes.
Lane 8: 7 seconds.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Other - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D

This product has been referenced in:

Connolly NP et al. Cross-species transcriptional analysis reveals conserved and host-specific neoplastic processes in mammalian glioma. Sci Rep 8:1180 (2018). Read more (PubMed: 29352201) »
Zhu QN et al. LncRNA H19 is a major mediator of doxorubicin chemoresistance in breast cancer cells through a cullin4A-MDR1 pathway. Oncotarget 8:91990-92003 (2017). Read more (PubMed: 29190892) »

See all 15 Publications for this product

Publishing research using ab52934? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Testing dicount for ab52934 in ChIP.

Abcam community

Verified customer

Asked on Nov 04 2011

Answer

DISCOUNT CODE: ****** Expiration date: ****** I am very pleased to hear you would like to accept our offer and test ab52934 in ChIP]. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for ChIPand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Abcam Scientific Support

Answered on Nov 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Colon ...)

Specification

Colon ...

Fixative

Paraformaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0

Permeabilization

Yes - Wash Buffer from DAKO

Mr. Rudolf Jung

Verified customer

Submitted Mar 18 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Overview

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References

This product has been referenced in:

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Topoisomerase II alpha antibody [EP1102Y]