Recombinant
RabMAb

Recombinant Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

Reviews (1)Q&A (1)References (29)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1102Y] to Topoisomerase II alpha
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Topoisomerase II alpha antibody [EP1102Y]
    See all Topoisomerase II alpha primary antibodies

  • Description

    Rabbit monoclonal [EP1102Y] to Topoisomerase II alpha

  • Host species

    Rabbit

  • Specificity

    Please be aware that the antibody provided positive results in cell lysate samples but negative results in tissue lysate samples.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Topoisomerase II alpha aa 1500-1600 (C terminal). The exact sequence is proprietary.
    Database link: P11388

  • Positive control

    • HeLa whole cell lysate (ab150035); human breast carcinoma tissue. WB: HeLa, Jurkat, Neuro-2a and PC-12 whole cell lysate; Human breast cancer, Mouse thymus lysate, Rat thymus lysate and Rat testis lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab52934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.

    • Target

    • Function

      Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.

    • Sequence similarities

      Belongs to the type II topoisomerase family.

    • Post-translational
      modifications

      Phosphorylation has no effect on catalytic activity.

    • Cellular localization

      Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • alpha isozyme antibody
      • ATP hydrolyzing DNA topoisomerase II alfa antibody
      • DNA gyrase antibody
      • DNA topoisomerase (ATP hydrolyzing) antibody
      • DNA topoisomerase 2 alpha antibody
      • DNA topoisomerase 2-alpha antibody
      • DNA topoisomerase II 170 kD antibody
      • DNA topoisomerase II alpha isozyme antibody
      • DNA topoisomerase II antibody
      • DNA Topoisomerase2 antibody
      • TOP 2A antibody
      • TOP2 antibody
      • TOP2A antibody
      • TOP2A_HUMAN antibody
      • Topoisomerase DNA II alpha 170kDa antibody
      • TP2A antibody
      see all

    Images

    • Western blot - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)
      Western blot - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)
      All lanes : Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
      Lane 2 : Jurkat (human acute T cell leukemia) whole cell lysate
      Lane 3 : Neuro-2a (mouse neuroblastoma) whole cell lysate
      Lane 4 : Human breast cancer lysate
      Lane 5 : Mouse thymus lysate
      Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 7 : Rat thymus lysate
      Lane 8 : Rat testis lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size: 174 kDa
      Observed band size: 174 kDa



      Exposure time:

      Lanes 1-3: 1 second.
      Lanes 4-7: 3 minutes.
      Lane 8: 7 seconds.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Other - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)
      Other - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (29)

    Publishing research using ab52934? Please let us know so that we can cite the reference in this datasheet.

    ab52934 has been referenced in 29 publications.

    • Wu J  et al. Long noncoding RNA UCA1 targets miR-582-5p and contributes to the progression and drug resistance of bladder cancer cells through ATG7-mediated autophagy inhibition. Onco Targets Ther 12:495-508 (2019). PubMed: 30666128
    • Xu S  et al. Upregulation of microRNA-194-5p inhibits hypopharyngeal carcinoma cell proliferation, migration and invasion by targeting SMURF1 via the mTOR signaling pathway. Int J Oncol 54:1245-1255 (2019). PubMed: 30720112
    • Holmes TH & Winn LM DNA Damage and Perturbed Topoisomerase IIa as a Target of 1,4-Benzoquinone Toxicity in Murine Fetal Liver Cells. Toxicol Sci N/A:N/A (2019). PubMed: 31340051
    • Zeng S  et al. Prognostic value of TOP2A in bladder urothelial carcinoma and potential molecular mechanisms. BMC Cancer 19:604 (2019). PubMed: 31216997
    • Adams CL  et al. Identification of a novel diagnostic gene expression signature to discriminate uterine leiomyoma from leiomyosarcoma. Exp Mol Pathol 110:104284 (2019). PubMed: 31301306
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Testing dicount for ab52934 in ChIP.

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    Answer

    DISCOUNT CODE: ****** Expiration date: ****** I am very pleased to hear you would like to accept our offer and test ab52934 in ChIP]. This code will give you 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for ChIPand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Topoisomerase II alpha antibody [EP1102Y]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Colon ...)
    Specification
    Colon ...
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization
    Yes - Wash Buffer from DAKO
    Read More

    Mr. Rudolf Jung

    Verified customer

    Submitted Mar 18 2011

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