Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)
- Datasheet
- Specific References (15)
- Protocols
Overview
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Product nameAnti-Topoisomerase II alpha antibody [EP1102Y]
See all Topoisomerase II alpha primary antibodies -
DescriptionRabbit monoclonal [EP1102Y] to Topoisomerase II alpha
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Host speciesRabbit
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SpecificityPlease be aware that the antibody provided positive results in cell lysate samples but negative results in tissue lysate samples.
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Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Topoisomerase II alpha (C terminal). The exact sequence is proprietary.
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Positive control
- HeLa cell lysate; human breast carcinoma tissue. WB: Hela, Jurkat, Neuro-2a and PC-12 whole cell lysate; Human breast cancer, Mouse thymus lysate, Rat thymus lysate and Rat testis lysate
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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Dissociation constant (KD)KD = 5.39 x 10 -10 M Learn more about KD
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEP1102Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab52934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa). | |
| IP | 1/50. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionControl of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
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Sequence similaritiesBelongs to the type II topoisomerase family.
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Post-translational
modificationsPhosphorylation has no effect on catalytic activity. -
Cellular localizationCytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 7153 Human
- Entrez Gene: 21973 Mouse
- Entrez Gene: 360243 Rat
- Omim: 126430 Human
- SwissProt: P11388 Human
- SwissProt: Q01320 Mouse
- SwissProt: P41516 Rat
- Unigene: 156346 Human
see all -
Alternative names
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
see all
Images
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![Western blot - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)](https://www.abcam.com/ps/products/52/ab52934/Images/ab52934-251091-ab52934wb.png)
Western blot - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)All lanes : Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (human acute T cell leukemia) whole cell lysate
Lane 3 : Neuro-2a (mouse neuroblastoma) whole cell lysate
Lane 4 : Human breast cancer lysate
Lane 5 : Mouse thymus lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 7 : Rat thymus lysate
Lane 8 : Rat testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 174 kDa
Observed band size: 174 kDaExposure time:
Lanes 1-3: 1 second.
Lanes 4-7: 3 minutes.
Lane 8: 7 seconds.Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)](https://www.abcam.com/ps/products/52/ab52934/Images/ab52934-251093-ab52934IHCPHumantestis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)](https://www.abcam.com/ps/products/52/ab52934/Images/ab52934-251092-ab52934IHCPHumancolon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)](https://www.abcam.com/ps/products/52/ab52934/Images/ab52934-251094-ab52934IHCPMousetestis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)](https://www.abcam.com/ps/products/52/ab52934/Images/ab52934-251095-ab52934IHCPMousecolon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)](https://www.abcam.com/ps/products/52/ab52934/Images/ab52934-251096-ab52934IHCPRattestis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)](https://www.abcam.com/ps/products/52/ab52934/Images/ab52934-251097-ab52934IHCPRatcolon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Topoisomerase II alpha with ab52934 at a dilution of 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Other - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)](https://www.abcam.com/ps/products/52/ab52934/Images/Topoisomerase-II-alpha-Primary-antibodies-ab52934-4.jpg)
Other - Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934)
References
This product has been referenced in:
- Connolly NP et al. Cross-species transcriptional analysis reveals conserved and host-specific neoplastic processes in mammalian glioma. Sci Rep 8:1180 (2018). Read more (PubMed: 29352201) »
- Zhu QN et al. LncRNA H19 is a major mediator of doxorubicin chemoresistance in breast cancer cells through a cullin4A-MDR1 pathway. Oncotarget 8:91990-92003 (2017). Read more (PubMed: 29190892) »
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