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    topoisomerase-ii-alpha-antibody-top2a1362-ab219321.pdf

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Epigenetics and Nuclear Signaling Chromosome Structure Scaffold Proteins
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Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
  • Immunocytochemistry - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
  • Western blot - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
  • Protein Array - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)

Key features and details

  • Mouse monoclonal [TOP2A/1362] to Topoisomerase II alpha
  • Suitable for: IHC-P, Protein Array, WB, ICC
  • Reacts with: Human
  • Isotype: IgG2b

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Anti-Topoisomerase II beta/TOP2B antibody (ab72334)

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Overview

  • Product name

    Anti-Topoisomerase II alpha antibody [TOP2A/1362]
    See all Topoisomerase II alpha primary antibodies
  • Description

    Mouse monoclonal [TOP2A/1362] to Topoisomerase II alpha
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Protein Array, WB, ICCmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Topoisomerase II alpha aa 1352-1493. The exact sequence is proprietary.
    Database link: P11388

  • Positive control

    • IHC-P: Human tonsil and bladder carcinoma tissue. ICC: HeLa cells. WB: HeLa cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab219321 was purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    TOP2A/1362
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Scaffold Proteins
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Topoisomerases
    • Cancer
    • Drug resistance
    • Topoisomerases
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP kits

Associated products

  • Alternative Versions

    • Anti-Topoisomerase II alpha antibody [TOP2A/1362] - BSA and Azide free (ab269765)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Human Topoisomerase II alpha protein (ab159735)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab219321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 min at RT).

Protein Array
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 174 kDa.
ICC
Use a concentration of 1 - 2 µg/ml.
Notes
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 min at RT).

Protein Array
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 174 kDa.
ICC
Use a concentration of 1 - 2 µg/ml.

Target

  • Function

    Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
  • Sequence similarities

    Belongs to the type II topoisomerase family.
  • Post-translational
    modifications

    Phosphorylation has no effect on catalytic activity.
  • Cellular localization

    Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
  • Target information above from: UniProt accession P11388 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7153 Human
    • Omim: 126430 Human
    • SwissProt: P11388 Human
    • Unigene: 156346 Human
    • Alternative names

      • alpha isozyme antibody
      • ATP hydrolyzing DNA topoisomerase II alfa antibody
      • DNA gyrase antibody
      • DNA topoisomerase (ATP hydrolyzing) antibody
      • DNA topoisomerase 2 alpha antibody
      • DNA topoisomerase 2-alpha antibody
      • DNA topoisomerase II 170 kD antibody
      • DNA topoisomerase II alpha isozyme antibody
      • DNA topoisomerase II antibody
      • DNA Topoisomerase2 antibody
      • TOP 2A antibody
      • TOP2 antibody
      • TOP2A antibody
      • TOP2A_HUMAN antibody
      • Topoisomerase DNA II alpha 170kDa antibody
      • TP2A antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Topoisomerase II alpha with ab219321 at 1 µg/ml.

    • Immunocytochemistry - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
      Immunocytochemistry - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)

      Immunocytochemistry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Topoisomerase II alpha with ab219321 at 1 µg/ml (Green). Phalloidin (Red) is used to label actin.

    • Western blot - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
      Western blot - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
      Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321) at 1 µg/ml + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

      Predicted band size: 174 kDa



      Western blot analysis of HeLa cell lysate labeling Topoisomerase II alpha with ab219321 at 1 ug/ml.

    • Protein Array - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
      Protein Array - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
      ab219321 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder carcinoma tissue labeling Topoisomerase II alpha with ab219321 at 1 µg/ml.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab219321? Please let us know so that we can cite the reference in this datasheet.

    ab219321 has not yet been referenced specifically in any publications.

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