Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
Key features and details
- Mouse monoclonal [TOP2A/1362] to Topoisomerase II alpha
- Suitable for: IHC-P, Protein Array, WB, ICC
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Topoisomerase II alpha antibody [TOP2A/1362]
See all Topoisomerase II alpha primary antibodies -
Description
Mouse monoclonal [TOP2A/1362] to Topoisomerase II alpha -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Protein Array, WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Topoisomerase II alpha aa 1352-1493. The exact sequence is proprietary.
Database link: P11388 -
Positive control
- IHC-P: Human tonsil and bladder carcinoma tissue. ICC: HeLa cells. WB: HeLa cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab219321 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
TOP2A/1362 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(Primary incubation for 30 min at RT). |
|
Protein Array |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 174 kDa.
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ICC |
Use a concentration of 1 - 2 µg/ml.
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Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (Primary incubation for 30 min at RT). |
Protein Array
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 174 kDa. |
ICC
Use a concentration of 1 - 2 µg/ml. |
Target
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Function
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. -
Sequence similarities
Belongs to the type II topoisomerase family. -
Post-translational
modificationsPhosphorylation has no effect on catalytic activity. -
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7153 Human
- Omim: 126430 Human
- SwissProt: P11388 Human
- Unigene: 156346 Human
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Alternative names
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling Topoisomerase II alpha with ab219321 at 1 µg/ml.
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Immunocytochemistry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Topoisomerase II alpha with ab219321 at 1 µg/ml (Green). Phalloidin (Red) is used to label actin.
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Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321) at 1 µg/ml + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 174 kDaWestern blot analysis of HeLa cell lysate labeling Topoisomerase II alpha with ab219321 at 1 ug/ml.
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ab219321 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha antibody [TOP2A/1362] (ab219321)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder carcinoma tissue labeling Topoisomerase II alpha with ab219321 at 1 µg/ml.
Datasheets and documents
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Datasheet download
References (0)
ab219321 has not yet been referenced specifically in any publications.