Recombinant
RabMAb

Recombinant Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765)

Overview

  • Product name
    Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722]
    See all Topoisomerase II alpha primary antibodies
  • Description
    Rabbit monoclonal [EP2722] to Topoisomerase II alpha (phospho S1106)
  • Host species
    Rabbit
  • Specificity
    ab75765 detects Topoisomerase II alpha phosphorylated on Serine 1106.
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Serine 1106 of human Topoisormerase II alpha

  • Positive control
    • HeLa whole cell lysate (ab150035), untreated or treated with AP and colonic adenocarcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab75765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
    • Sequence similarities
      Belongs to the type II topoisomerase family.
    • Post-translational
      modifications
      Phosphorylation has no effect on catalytic activity.
    • Cellular localization
      Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • alpha isozyme antibody
      • ATP hydrolyzing DNA topoisomerase II alfa antibody
      • DNA gyrase antibody
      • DNA topoisomerase (ATP hydrolyzing) antibody
      • DNA topoisomerase 2 alpha antibody
      • DNA topoisomerase 2-alpha antibody
      • DNA topoisomerase II 170 kD antibody
      • DNA topoisomerase II alpha isozyme antibody
      • DNA topoisomerase II antibody
      • DNA Topoisomerase2 antibody
      • TOP 2A antibody
      • TOP2 antibody
      • TOP2A antibody
      • TOP2A_HUMAN antibody
      • Topoisomerase DNA II alpha 170kDa antibody
      • TP2A antibody
      see all

    Images

    • All lanes : Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765) at 1/500 dilution

      Lane 1 : HeLa cell lysates, untreated
      Lane 2 : HeLa cell lysates, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 174 kDa
      Observed band size: 174 kDa

    • ab75765 at 1/100 dilution staining Topoisomerase II alpha in colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue

    References

    This product has been referenced in:
    • Bobustuc GC  et al. MGMT inhibition in ER positive breast cancer leads to CDC2, TOP2A, AURKB, CDC20, KIF20A, Cyclin A2, Cyclin B2, Cyclin D1, ERa and Survivin inhibition and enhances response to temozolomide. Oncotarget 9:29727-29742 (2018). Read more (PubMed: 30038716) »
    • Vega SL  et al. High-content image informatics of the structural nuclear protein NuMA parses trajectories for stem/progenitor cell lineages and oncogenic transformation. Exp Cell Res 351:11-23 (2017). Read more (PubMed: 28034673) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab75765.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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