Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2722] to Topoisomerase II alpha (phospho S1106)
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-Topoisomerase II alpha (phospho S1106) antibody [EP2722]
See all Topoisomerase II alpha primary antibodies
DescriptionRabbit monoclonal [EP2722] to Topoisomerase II alpha (phospho S1106)
Specificityab75765 detects Topoisomerase II alpha phosphorylated on Serine 1106.
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
A phospho specific peptide corresponding to residues surrounding Serine 1106 of human Topoisormerase II alpha
- HeLa whole cell lysate (ab150035), untreated or treated with AP and colonic adenocarcinoma tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionControl of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
Sequence similaritiesBelongs to the type II topoisomerase family.
modificationsPhosphorylation has no effect on catalytic activity.
Cellular localizationCytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
- Information by UniProt
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
All lanes : Anti-Topoisomerase II alpha (phospho S1106) antibody [EP2722] (ab75765) at 1/500 dilution
Lane 1 : HeLa cell lysates, untreated
Lane 2 : HeLa cell lysates, treated with AP
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 174 kDa
Observed band size: 174 kDa
ab75765 at 1/100 dilution staining Topoisomerase II alpha in colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab75765 has been referenced in 4 publications.
- Bobustuc GC et al. MGMT inhibition in ER positive breast cancer leads to CDC2, TOP2A, AURKB, CDC20, KIF20A, Cyclin A2, Cyclin B2, Cyclin D1, ERa and Survivin inhibition and enhances response to temozolomide. Oncotarget 9:29727-29742 (2018). PubMed: 30038716
- Huang CT et al. A Large-Scale Gene Expression Intensity-Based Similarity Metric for Drug Repositioning. iScience 7:40-52 (2018). PubMed: 30267685
- Vega SL et al. High-content image informatics of the structural nuclear protein NuMA parses trajectories for stem/progenitor cell lineages and oncogenic transformation. Exp Cell Res 351:11-23 (2017). PubMed: 28034673
- Wartlick F et al. DNA damage response (DDR) induced by topoisomerase II poisons requires nuclear function of the small GTPase Rac. Biochim Biophys Acta 1833:3093-3103 (2013). WB ; Human . PubMed: 23999236