Anti-Topoisomerase II alpha (phospho S1525) antibody (ab138683)

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Western blot - Anti-Topoisomerase II alpha (phospho S1525) antibody (ab138683)

    Key features and details

    • Rabbit polyclonal to Topoisomerase II alpha (phospho S1525)
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    Overview

    • Product name

      Anti-Topoisomerase II alpha (phospho S1525) antibody
      See all Topoisomerase II alpha primary antibodies

    • Description

      Rabbit polyclonal to Topoisomerase II alpha (phospho S1525)

    • Host species

      Rabbit

    • Specificity

      ab138683 detects endogenous levels of Topoisomerase II alpha only when phosphorylated at S1525.

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Rat, Human

    • Immunogen

      Synthetic phosphopeptide derived from the C-terminal region of Human Topoisomerase II alpha around the phosphorylation site of S1525.

    • Positive control

      • RAW 264.7 cell extract, treated with TNF (20ng/ml, 5 mins).

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab138683 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/1000. Predicted molecular weight: 174 kDa.

    Target

    • Function

      Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.

    • Sequence similarities

      Belongs to the type II topoisomerase family.

    • Post-translational
      modifications

      Phosphorylation has no effect on catalytic activity.

    • Cellular localization

      Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • alpha isozyme antibody
      • ATP hydrolyzing DNA topoisomerase II alfa antibody
      • DNA gyrase antibody
      • DNA topoisomerase (ATP hydrolyzing) antibody
      • DNA topoisomerase 2 alpha antibody
      • DNA topoisomerase 2-alpha antibody
      • DNA topoisomerase II 170 kD antibody
      • DNA topoisomerase II alpha isozyme antibody
      • DNA topoisomerase II antibody
      • DNA Topoisomerase2 antibody
      • TOP 2A antibody
      • TOP2 antibody
      • TOP2A antibody
      • TOP2A_HUMAN antibody
      • Topoisomerase DNA II alpha 170kDa antibody
      • TP2A antibody
      see all

    Images

    • Western blot - Anti-Topoisomerase II alpha (phospho S1525) antibody (ab138683)
      Western blot - Anti-Topoisomerase II alpha (phospho S1525) antibody (ab138683)
      Anti-Topoisomerase II alpha (phospho S1525) antibody (ab138683) at 1/500 dilution + RAW 264.7 cell extracts, treated with TNF (20ng/ml, 5 mins) at 30 µg

      Predicted band size: 174 kDa

    References (1)

    Publishing research using ab138683? Please let us know so that we can cite the reference in this datasheet.

    ab138683 has been referenced in 1 publication.

    • Yu L  et al. Longitudinal Analysis of Gene Expression Changes During Cervical Carcinogenesis Reveals Potential Therapeutic Targets. Evol Bioinform Online 16:1176934320920574 (2020). PubMed: 32489245

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