Key features and details
- Rabbit polyclonal to Topoisomerase II alpha (phospho S1525)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Topoisomerase II alpha (phospho S1525) antibody
See all Topoisomerase II alpha primary antibodies
DescriptionRabbit polyclonal to Topoisomerase II alpha (phospho S1525)
Specificityab138683 detects endogenous levels of Topoisomerase II alpha only when phosphorylated at S1525.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
Synthetic phosphopeptide derived from the C-terminal region of Human Topoisomerase II alpha around the phosphorylation site of S1525.
- RAW 264.7 cell extract, treated with TNF (20ng/ml, 5 mins).
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 49% PBS
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab138683 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Our Abpromise guarantee covers the use of ab138683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 174 kDa.|
FunctionControl of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
Sequence similaritiesBelongs to the type II topoisomerase family.
modificationsPhosphorylation has no effect on catalytic activity.
Cellular localizationCytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
- Information by UniProt
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
ab138683 has been referenced in 1 publication.
- Yu L et al. Longitudinal Analysis of Gene Expression Changes During Cervical Carcinogenesis Reveals Potential Therapeutic Targets. Evol Bioinform Online 16:1176934320920574 (2020). PubMed: 32489245