Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
- Suitable for: IHC-P, WB, ICC
- Reacts with: Human
Product nameAnti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]
See all Topoisomerase II alpha primary antibodies
DescriptionRabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
Specificityab52853 only detects Topoisomerase II alpha phosphorylated on Threonine 1343.
Tested applicationsSuitable for: IHC-P, WB, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topoisomerase II alpha.
- WB: HeLa cell lysates. IHC-P: Human cervical carcinoma tissue. ICC: HeLa cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab52853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).|
|ICC||1/50 - 1/100.|
FunctionControl of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
Sequence similaritiesBelongs to the type II topoisomerase family.
modificationsPhosphorylation has no effect on catalytic activity.
Cellular localizationCytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
- Information by UniProt
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution
Lane 1 : HeLa cell lysate treated with AP
Lane 2 : HeLa cell lysate untreated
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 174 kDa
Observed band size: 174 kDa
ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Topoisomerase II alpha with purified ab52853 at 1/50 dilution (4 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.14 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab52853 has not yet been referenced specifically in any publications.