Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]
See all Topoisomerase II alpha primary antibodies -
DescriptionRabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
-
Host speciesRabbit
-
Specificityab52853 only detects Topoisomerase II alpha phosphorylated on Threonine 1343.
-
Tested applicationsSuitable for: IHC-P, WB, ICCmore details
Unsuitable for: Flow Cyt or IP -
Species reactivityReacts with: Human
-
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topoisomerase II alpha.
-
Positive control
- WB: HeLa cell lysates. IHC-P: Human cervical carcinoma tissue.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEP1054Y
-
IsotypeIgG
-
Research areas
Associated products
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab52853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa). | |
| ICC | 1/50 - 1/100. |
Target
-
FunctionControl of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
-
Sequence similaritiesBelongs to the type II topoisomerase family.
-
Post-translational
modificationsPhosphorylation has no effect on catalytic activity. -
Cellular localizationCytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
- Information by UniProt
-
Database links
- Entrez Gene: 7153 Human
- Omim: 126430 Human
- SwissProt: P11388 Human
- Unigene: 156346 Human
-
Alternative names
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
see all
Images
-
![Western blot - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)](https://www.abcam.com/ps/products/52/ab52853/Images/ab52853wb.gif)
Western blot - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution
Lane 1 : HeLa cell lysate treated with AP
Lane 2 : HeLa cell lysate untreated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 174 kDa
Observed band size: 174 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)](https://www.abcam.com/ps/products/52/ab52853/Images/ab52853ihc.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
Datasheets and documents
References
ab52853 has not yet been referenced specifically in any publications.
