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Epigenetics and Nuclear Signaling Chromosome Structure Scaffold Proteins
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RecombinantRabMAb

Recombinant Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)

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Western blot - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
  • Immunocytochemistry - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
  • Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
  • Suitable for: IHC-P, WB, ICC
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]
    See all Topoisomerase II alpha primary antibodies
  • Description

    Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysates. IHC-P: Human cervical carcinoma tissue. ICC: HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1054Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Scaffold Proteins
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • Topoisomerases
    • Cancer
    • Drug resistance
    • Topoisomerases

Associated products

  • Alternative Versions

    • Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] - BSA and Azide free (ab247333)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Topoisomerase II alpha protein (ab159735)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
ICC
1/50 - 1/100.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
ICC
1/50 - 1/100.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
  • Sequence similarities

    Belongs to the type II topoisomerase family.
  • Post-translational
    modifications

    Phosphorylation has no effect on catalytic activity.
  • Cellular localization

    Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
  • Target information above from: UniProt accession P11388 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7153 Human
    • Omim: 126430 Human
    • SwissProt: P11388 Human
    • Unigene: 156346 Human
    • Alternative names

      • alpha isozyme antibody
      • ATP hydrolyzing DNA topoisomerase II alfa antibody
      • DNA gyrase antibody
      • DNA topoisomerase (ATP hydrolyzing) antibody
      • DNA topoisomerase 2 alpha antibody
      • DNA topoisomerase 2-alpha antibody
      • DNA topoisomerase II 170 kD antibody
      • DNA topoisomerase II alpha isozyme antibody
      • DNA topoisomerase II antibody
      • DNA Topoisomerase2 antibody
      • TOP 2A antibody
      • TOP2 antibody
      • TOP2A antibody
      • TOP2A_HUMAN antibody
      • Topoisomerase DNA II alpha 170kDa antibody
      • TP2A antibody
      see all

    Images

    • Western blot - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
      Western blot - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
      All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution

      Lane 1 : HeLa cell lysate treated with AP
      Lane 2 : HeLa cell lysate untreated

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 174 kDa
      Observed band size: 174 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)

      ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
      Immunocytochemistry - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
      Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Topoisomerase II alpha with purified ab52853 at 1/50 dilution (4 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.14 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
      Negative control: PBS instead of the primary antibody.
    • Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
      Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab52853? Please let us know so that we can cite the reference in this datasheet.

    ab52853 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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