Product nameAnti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]
See all Topoisomerase II alpha primary antibodies
DescriptionRabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
Specificityab52853 only detects Topoisomerase II alpha phosphorylated on Threonine 1343.
Tested applicationsSuitable for: IHC-P, WB, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topoisomerase II alpha.
- WB: HeLa cell lysates. IHC-P: Human cervical carcinoma tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab52853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).|
|ICC||1/50 - 1/100.|
FunctionControl of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
Sequence similaritiesBelongs to the type II topoisomerase family.
modificationsPhosphorylation has no effect on catalytic activity.
Cellular localizationCytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
- Information by UniProt
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution
Lane 1 : HeLa cell lysate treated with AP
Lane 2 : HeLa cell lysate untreated
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 174 kDa
Observed band size: 174 kDa
ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab52853 has not yet been referenced specifically in any publications.