Recombinant Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)


  • Product name

    Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y]
    See all Topoisomerase II alpha primary antibodies
  • Description

    Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
  • Host species

  • Specificity

    ab52853 only detects Topoisomerase II alpha phosphorylated on Threonine 1343.
  • Tested applications

    Suitable for: IHC-P, WB, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topoisomerase II alpha.

  • Positive control

    • WB: HeLa cell lysates. IHC-P: Human cervical carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab52853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
    • Sequence similarities

      Belongs to the type II topoisomerase family.
    • Post-translational

      Phosphorylation has no effect on catalytic activity.
    • Cellular localization

      Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • alpha isozyme antibody
      • ATP hydrolyzing DNA topoisomerase II alfa antibody
      • DNA gyrase antibody
      • DNA topoisomerase (ATP hydrolyzing) antibody
      • DNA topoisomerase 2 alpha antibody
      • DNA topoisomerase 2-alpha antibody
      • DNA topoisomerase II 170 kD antibody
      • DNA topoisomerase II alpha isozyme antibody
      • DNA topoisomerase II antibody
      • DNA Topoisomerase2 antibody
      • TOP 2A antibody
      • TOP2 antibody
      • TOP2A antibody
      • TOP2A_HUMAN antibody
      • Topoisomerase DNA II alpha 170kDa antibody
      • TP2A antibody
      see all


    • All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution

      Lane 1 : HeLa cell lysate treated with AP
      Lane 2 : HeLa cell lysate untreated

      Lysates/proteins at 10 µg per lane.

      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 174 kDa
      Observed band size: 174 kDa

    • ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


    ab52853 has not yet been referenced specifically in any publications.

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