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Rabbit monoclonal [EP1054Y] to Topoisomerase II alpha (phospho T1343)
ab52853 only detects Topoisomerase II alpha phosphorylated on Threonine 1343.
, ICCmore detailsUnsuitable for:
Flow Cyt or IP
A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topoisomerase II alpha.
- WB: HeLa cell lysates.
IHC-P: Human cervical carcinoma tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use at an assay dependent concentration.
||1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
||1/50 - 1/100.
Is unsuitable for Flow Cyt or IP.
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
Belongs to the type II topoisomerase family.
Phosphorylation has no effect on catalytic activity.
Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
Information by UniProt
- alpha isozyme antibody
- ATP hydrolyzing DNA topoisomerase II alfa antibody
- DNA gyrase antibody
Western blot - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution
Lane 1 : HeLa cell lysate treated with AP
Lane 2 : HeLa cell lysate untreated
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 174 kDa
Observed band size: 174 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)
ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"