Overview

  • Product name

    Anti-Topoisomerase II beta/TOP2B antibody
    See all Topoisomerase II beta/TOP2B primary antibodies
  • Description

    Rabbit polyclonal to Topoisomerase II beta/TOP2B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Topoisomerase II beta/TOP2B aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab156303)

  • Positive control

    • WB: Wild-type NALM-6 cell lysate. HeLa and NIH/3T3 whole cell lysate. Jurkat, K562, MCF7 and HepG2 cell lysate.
  • General notes

     This product was previously labelled as Topoisomerase II beta

     

Properties

Applications

Our Abpromise guarantee covers the use of ab125297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 183 kDa (predicted molecular weight: 183 kDa). Abcam recommends using milk as the blocking agent - 5%

Target

  • Function

    Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • Sequence similarities

    Belongs to the type II topoisomerase family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen MLAA 44 antibody
    • beta isozyme antibody
    • DNA topoisomerase 2 beta antibody
    • DNA topoisomerase 2-beta antibody
    • DNA topoisomerase II 180 kD antibody
    • DNA topoisomerase II antibody
    • DNA topoisomerase II beta antibody
    • DNA topoisomerase II beta isozyme antibody
    • Top 2 antibody
    • TOP 2B antibody
    • TOP IIB antibody
    • Top2 beta antibody
    • TOP2B antibody
    • TOP2B_HUMAN antibody
    • Top2beta antibody
    • TOPIIB antibody
    • Topo II beta antibody
    • Topoisomerase (DNA) II beta 180kDa antibody
    • Topoisomerase (DNA) II beta antibody
    • Topoisomerase IIb antibody
    • U937 associated antigen antibody
    see all

Images

  • Lane 1: Wild-type NALM-6 cell lysate (20 µg)
    Lane 2: Topoisomerase II beta/TOP2B knockout NALM-6 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: NIH/3T3 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab125297 observed at 200 kDa. Red - loading control, ab7291, observed at 50 kDa.
    ab125297 was shown to recognize Topoisomerase II beta/TOP2B when Topoisomerase II beta/TOP2B knockout samples were used, along with additional cross-reactive bands. Wild-type and Topoisomerase II beta/TOP2B knockout samples were subjected to SDS-PAGE. ab125297 and ab7291 (loading control to alpha tubulin) were diluted 1 μg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-Topoisomerase II beta/TOP2B antibody (ab125297) at 1 µg/ml (Milk blocking - 5%)

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 183 kDa
    Observed band size: 183 kDa
    Additional bands at: 120 kDa, 150 kDa, 42 kDa, 55 kDa, 66 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    Abcam recommends using milk as the blocking agent - 5%. This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab125297 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:

  • Pennington SM  et al. Defective protein repair under methionine sulfoxide A deletion drives autophagy and ARE-dependent gene transcription. Redox Biol 16:401-413 (2018). WB . Read more (PubMed: 29649787) »
See 1 Publication for this product

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