Anti-Topoisomerase II beta/TOP2B antibody (ab235898)
Key features and details
- Rabbit polyclonal to Topoisomerase II beta/TOP2B
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Topoisomerase II beta/TOP2B antibody
See all Topoisomerase II beta/TOP2B primary antibodies -
Description
Rabbit polyclonal to Topoisomerase II beta/TOP2B -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chinese hamster -
Immunogen
Recombinant fragment corresponding to Human Topoisomerase II beta/TOP2B aa 1400 to the C-terminus.
Database link: Q02880-1 -
Positive control
- IHC-P: Human cervical cancer tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/20 - 1/200.
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IHC-P |
1/20 - 1/200.
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Notes |
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ICC/IF
1/20 - 1/200. |
IHC-P
1/20 - 1/200. |
Target
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Function
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. -
Sequence similarities
Belongs to the type II topoisomerase family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 7155 Human
- Entrez Gene: 21974 Mouse
- Omim: 126431 Human
- SwissProt: Q64399 Chinese hamster
- SwissProt: Q02880 Human
- SwissProt: Q64511 Mouse
- Unigene: 475733 Human
- Unigene: 130362 Mouse
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Alternative names
- Antigen MLAA 44 antibody
- beta isozyme antibody
- DNA topoisomerase 2 beta antibody
see all
Images
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Paraffin-embedded human cervical cancer tissue stained for Topoisimerase II beta using ab235898 at 1/100 dilution in immunohistochemical analysis.
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HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Topoisomerase II beta/TOP2B (green) using ab235898 at 1/100 dilution in ICC/IF. Secondary antibody is an Alexa-Fluor® 488-conjugated Goat Anti-Rabbit IgG(H+L).
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235898 has not yet been referenced specifically in any publications.