Product nameAnti-Topoisomerase II beta/TOP2B antibody
See all Topoisomerase II beta/TOP2B primary antibodies
DescriptionRabbit polyclonal to Topoisomerase II beta/TOP2B
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Topoisomerase II beta/TOP2B aa 1570-1621 (C terminal). The exact sequence is proprietary. NP_001059.2
Database link: Q02880
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human small cell lung cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264158 in the following tested applications.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 183 kDa.|
FunctionControl of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
Sequence similaritiesBelongs to the type II topoisomerase family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus > nucleolus.
- Information by UniProt
- Antigen MLAA 44 antibody
- beta isozyme antibody
- DNA topoisomerase 2 beta antibody
All lanes : Anti-Topoisomerase II beta/TOP2B antibody (ab264158) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 183 kDa
Exposure time: 30 seconds
Topoisomerase II beta/TOP2B was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264158 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264158 at 0.1 µg/ml.
Lane 1: ab264158 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 10 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small cell lung cancer tissue labeling Topoisomerase II beta/TOP2B with ab264158 at 1/200 dilution. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ab264158 has not yet been referenced specifically in any publications.