• Product name

    Anti-Topoisomerase II beta/TOP2B antibody
    See all Topoisomerase II beta/TOP2B primary antibodies
  • Description

    Rabbit polyclonal to Topoisomerase II beta/TOP2B
  • Host species

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human Topoisomerase II beta/TOP2B.

  • Positive control

    • Whole cell lysate from Hela and 293T cells.
  • General notes

     This product was previously labelled as Topoisomerase II beta




Our Abpromise guarantee covers the use of ab72334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 210 kDa (predicted molecular weight: 183 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function

    Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • Sequence similarities

    Belongs to the type II topoisomerase family.
  • Post-translational

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen MLAA 44 antibody
    • beta isozyme antibody
    • DNA topoisomerase 2 beta antibody
    • DNA topoisomerase 2-beta antibody
    • DNA topoisomerase II 180 kD antibody
    • DNA topoisomerase II antibody
    • DNA topoisomerase II beta antibody
    • DNA topoisomerase II beta isozyme antibody
    • Top 2 antibody
    • TOP 2B antibody
    • TOP IIB antibody
    • Top2 beta antibody
    • TOP2B antibody
    • TOP2B_HUMAN antibody
    • Top2beta antibody
    • TOPIIB antibody
    • Topo II beta antibody
    • Topoisomerase (DNA) II beta 180kDa antibody
    • Topoisomerase (DNA) II beta antibody
    • Topoisomerase IIb antibody
    • U937 associated antigen antibody
    see all


  • All lanes : Anti-Topoisomerase II beta/TOP2B antibody (ab72334) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 183 kDa
    Observed band size: 210 kDa
    why is the actual band size different from the predicted?

    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Topoisomerase II beta/TOP2B with ab72334 at 1/200 (1µg/ml). Detection: DAB.

  • Immunoprecipitation/ Western Blot of Topoisomerase II beta/TOP2B. Lane 1: ab72334 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72334 at 0.1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 30 seconds.

  • IHC image of ab72334 staining in human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72334, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Bansal K  et al. The transcriptional regulator Aire binds to and activates super-enhancers. Nat Immunol 18:263-273 (2017). Read more (PubMed: 28135252) »
  • Yoshida H  et al. Brd4 bridges the transcriptional regulators, Aire and P-TEFb, to promote elongation of peripheral-tissue antigen transcripts in thymic stromal cells. Proc Natl Acad Sci U S A 112:E4448-57 (2015). WB . Read more (PubMed: 26216992) »
See all 2 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Human Cell lysate - nuclear (Colorectal cancer cell lines)
Negative control
Colorectal cancer cell lines
Detection step
Real-time PCR
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
Cells were treated or not with 1uM of DNA damaging agent to induce DNA double strand breaks.

Abcam user community

Verified customer

Submitted Jul 24 2018


I am very pleased to hear you would like to accept our offer and test ab58442 and ab72334 together in sELISA. These codes will give you 2 free primary antibodies before the expiration date. To redeem this offer, please submit an Abreview for sELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The codes will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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Merci de nous avoir contactés. Comme convenu j'ai procédé au remplacement de ab72334 par une unité de ab58442. Le numéro de commande de remplacement gratuit est 979924. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition. N'hésitez pas à nous contacter lors d'une prochaine occasion.

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Bonjour, Malheureusement le laboratoire qui produit l'anticorps ab72334 ne dispose pas de lots différents de celui que vous avez reçu dernièrement. Est-ce qu'un remplacement avec un anticorps alternatif vous conviendrait? Nous avons : ab58442, polyclonal de lapin ChIP grade, www.abcam.com/ab58442 ab109524, monoclonal de lapin, www.abcam.com/ab109524 Nous pouvons également procéder à l'édition d'un avoir/remboursement. Merci de m'indiquer la résolution qui vous conviendrait le mieux.  

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Merci de nous avoir contactés et de nous avoir fournis tous ces détails. Nous allons investiguer le problème rencontré avec ce lot. Je suis désolé que le dernier lot de ab72334 que vous avez reçu ne fonctionne pas comme attendu. Je serai heureux de procéder au plus vite au remplacement de ce produit. Cependant, cet anticorps n'est plus en stock. Je vais contacter le laboratoire afin de m'assurer que le prochain lot que nous aurons en stock sera différent de celui qui vous a posé problème. Merci de votre patience.

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