Anti-Topoisomerase III alpha antibody (ab108493)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Topoisomerase III alpha antibody -
Description
Rabbit polyclonal to Topoisomerase III alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla
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Immunogen
Synthetic peptide corresponding to Human Topoisomerase III alpha aa 900-1000 conjugated to keyhole limpet haemocyanin.
(Peptide available asab139195) -
Positive control
- This antibody gave a positive signal in both HeLa and HEK293 whole cell lysates. This antibody gave a positive result in IF in the following Methanol fixed cell line: HeLa.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab108493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 112 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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Function
Reduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. -
Tissue specificity
High expression is found in testis, heart, skeletal muscle and pancreas. -
Sequence similarities
Belongs to the prokaryotic type I/III topoisomerase family.
Contains 1 Toprim domain. - Information by UniProt
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Database links
- Entrez Gene: 7156 Human
- Omim: 601243 Human
- SwissProt: Q13472 Human
- Unigene: 592115 Human
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Alternative names
- DNA topoisomerase 3 alpha antibody
- DNA topoisomerase 3-alpha antibody
- DNA topoisomerase III alpha antibody
see all
Images
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Western blot - Anti-Topoisomerase III alpha antibody (ab108493)All lanes : Anti-Topoisomerase III alpha antibody (ab108493) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase III alpha antibody (ab108493)ab108493 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108493 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Datasheets and documents
References
ab108493 has not yet been referenced specifically in any publications.
