Anti-Topoisomerase III alpha antibody (ab108493)
Key features and details
- Rabbit polyclonal to Topoisomerase III alpha
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Topoisomerase III alpha antibody -
Description
Rabbit polyclonal to Topoisomerase III alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla
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Immunogen
Synthetic peptide corresponding to Human Topoisomerase III alpha aa 900-1000 conjugated to keyhole limpet haemocyanin.
(Peptide available asab139195) -
Positive control
- This antibody gave a positive signal in both HeLa and HEK293 whole cell lysates. This antibody gave a positive result in IF in the following Methanol fixed cell line: HeLa.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab108493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 112 kDa). | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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Function
Reduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. -
Tissue specificity
High expression is found in testis, heart, skeletal muscle and pancreas. -
Sequence similarities
Belongs to the prokaryotic type I/III topoisomerase family.
Contains 1 Toprim domain. - Information by UniProt
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Database links
- Entrez Gene: 7156 Human
- Omim: 601243 Human
- SwissProt: Q13472 Human
- Unigene: 592115 Human
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Alternative names
- DNA topoisomerase 3 alpha antibody
- DNA topoisomerase 3-alpha antibody
- DNA topoisomerase III alpha antibody
see all
Images
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Western blot - Anti-Topoisomerase III alpha antibody (ab108493)All lanes : Anti-Topoisomerase III alpha antibody (ab108493) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase III alpha antibody (ab108493)ab108493 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108493 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (0)
ab108493 has not yet been referenced specifically in any publications.
