Anti-Topoisomerase III alpha antibody (ab108493)

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Overview

  • Product name
    Anti-Topoisomerase III alpha antibody
  • Description
    Rabbit polyclonal to Topoisomerase III alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human Topoisomerase III alpha.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • This antibody gave a positive signal in both HeLa and HEK293 whole cell lysates. This antibody gave a positive result in IF in the following Methanol fixed cell line: HeLa.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 112 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Reduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells.
  • Tissue specificity
    High expression is found in testis, heart, skeletal muscle and pancreas.
  • Sequence similarities
    Belongs to the prokaryotic type I/III topoisomerase family.
    Contains 1 Toprim domain.
  • Information by UniProt
  • Database links
    • Alternative names
      • DNA topoisomerase 3 alpha antibody
      • DNA topoisomerase 3-alpha antibody
      • DNA topoisomerase III alpha antibody
      • TOP 3 antibody
      • TOP3 antibody
      • TOP3A antibody
      • TOP3A_HUMAN antibody
      • topo III-alpha antibody
      • topoisomerase (DNA) III alpha antibody
      • topoisomerase (DNA) III antibody
      • topoisomerase III alpha antibody
      • ZGRF7 antibody
      • zinc finger, GRF-type containing 7 antibody
      see all

    Images

    • Western blot - Anti-Topoisomerase III alpha antibody (ab108493)
      Western blot - Anti-Topoisomerase III alpha antibody (ab108493)
      All lanes : Anti-Topoisomerase III alpha antibody (ab108493) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
      Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 112 kDa
      Observed band size: 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 110 kDa, 70 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 16 minutes
    • Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase III alpha antibody (ab108493)
      Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase III alpha antibody (ab108493)

      ab108493 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108493 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    ab108493 has not yet been referenced specifically in any publications.

    Publishing research using ab108493? Please let us know so that we can cite the reference in this datasheet.

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