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Rabbit polyclonal to TOR1AIP1
Synthetic peptide, corresponding to a region between residues 175 and 225 of human TOR1AIP1 (NP_056417.2).
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab86307 was affinity purified using an epitope specific to TOR1AIP1 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5 µg/mg of lysate.
WB: 1/2000 - 1/10,000. Predicted molecular weight: 66 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Binds to A- and B-type lamins. Possible role in membrane attachment and assembly of the nuclear lamina.
Belongs to the TOR1AIP family.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus inner membrane.
Information by UniProt
Lamin-associated protein 1B antibody
Lamina associated polypeptide 1B antibody
Lamina associated polypeptide 1C antibody
Western blot - Anti-TOR1AIP1 antibody (ab86307)
All lanes : Anti-TOR1AIP1 antibody (ab86307) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-TOR1AIP1 antibody (ab86307)
Detection of TOR1AIP1 by Western Blot of Immunprecipitate.
ab86307, at 1 µg/ml, staining TOR1AIP1 in HeLa whole cell lysate immunoprecipitated using ab86307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with an exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"