Key features and details
- Rabbit polyclonal to TOR1AIP1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TOR1AIP1 antibody
DescriptionRabbit polyclonal to TOR1AIP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to a region between residues 175 and 225 of human TOR1AIP1 (NP_056417.2).
- WB: Jurkat nuclear extract lysate (ab14844), HeLa and 293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab86307 was affinity purified using an epitope specific to TOR1AIP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab86307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 66 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionBinds to A- and B-type lamins. Possible role in membrane attachment and assembly of the nuclear lamina.
Sequence similaritiesBelongs to the TOR1AIP family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus inner membrane.
- Information by UniProt
- Lamin-associated protein 1B antibody
- Lamina associated polypeptide 1B antibody
- Lamina associated polypeptide 1C antibody
All lanes : Anti-TOR1AIP1 antibody (ab86307) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 30 seconds
Detection of TOR1AIP1 by Western Blot of Immunprecipitate.
ab86307, at 1 µg/ml, staining TOR1AIP1 in HeLa whole cell lysate immunoprecipitated using ab86307 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab86307 has not yet been referenced specifically in any publications.