Overview

  • Product name

  • Description

    Rabbit polyclonal to TORC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Guinea pig, Dog, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to C terminal amino acids 630-634 of Human MECT1 (NP_056136.1).

  • Positive control

    • Human Breast Carcinoma, Colon Carcinoma, Non-Small Cell Lung Cancer, Prostate Carcinoma, Testicular Seminoma and Mouse Brain, Renal Cell Carcinoma, Teratoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab114974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.

Target

  • Function

    Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock.
  • Tissue specificity

    Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney.
  • Involvement in disease

    A chromosomal aberration involving CRTC1 is found in mucoepidermoid carcinomas, benign Warthin tumors and clear cell hidradenomas. Translocation t(11;19)(q21;p13) with MAML2. The fusion protein consists of the N-terminus of CRTC1 joined to the C-terminus of MAML2. The reciprocal fusion protein consisting of the N-terminus of MAML2 joined to the C-terminus of CRTC1 has been detected in a small number of mucoepidermoid carcinomas.
  • Sequence similarities

    Belongs to the TORC family.
  • Post-translational
    modifications

    Phosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels (PubMed:15589160). Light stimulation triggers a nuclear accumulation in the suprachiasmatic nucleus (SCN) of the brain (By similarity).
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0616 antibody
    • CREB regulated transcription coactivator 1 antibody
    • CREB-regulated transcription coactivator 1 antibody
    • CRTC1 antibody
    • CRTC1_HUMAN antibody
    • FLJ14027 antibody
    • MECT 1 antibody
    • Mucoepidermoid carcinoma translocated 1 antibody
    • Mucoepidermoid carcinoma translocated protein 1 antibody
    • TORC-1 antibody
    • TORC1 antibody
    • Transducer of CREB protein 1 antibody
    • Transducer of regulated cAMP response element binding protein 1 antibody
    • Transducer of regulated cAMP response element-binding protein (CREB) 1 antibody
    • Transducer of regulated cAMP response element-binding protein 1 antibody
    • WAMTP1 antibody
    see all

Images

  • ab114974 at 1/250 dilution staining MECT1 in Formalin-fixed, Paraffin-embedded Human Lung carcinoma tissue by Immunohistochemistry.
    Detection: DAB staining.
  • ab114974 at 1/250 dilution staining MECT1 in Formalin-fixed, Paraffin-embedded Mouse Brain tissue by Immunohistochemistry.
    Detection: DAB staining.

References

ab114974 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry and your interest in our products.

We are not aware if any of our antibodies to TORC1 (CRTC1) have been involved in such scientific investigations. If they were, then the publication list of the on-line datasheet would have the specific scientific paper cited.

Currently, we have a few antibodies (TORC1) which have been tested for IHC-P such as ab109736, ab114974, ab115330 but we do not have any information regarding their suitability for FISH.

However, the good news is that we have a special offer for rabbit monoclonal antibodies (Rabmab) in November and ab109736 is a Rabmab so it would apply for this promotion (free vial). You may wish to discuss it with your customer and read the follwoing webpage for further details.

https://www.abcam.com/index.html?pageconfig=resource&rid=15447&viapagetrap=freerabmab

If you need any further assistance in the future, please do not hesitate to contact me.

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